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Transient Induction of Gene Expression in the Nervous System in Response to Extracellular Signals

  • Harvey R. Herschman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 265)

Abstract

My laboratory has, for a number of years, been interested in the molecular mechanisms by which extracellular effectors such as growth factors and hormones mediate biological responses in target cells. We have utilized both genetic approaches to this problem and, more recently, molecular approaches. Our genetic studies have been concerned primarily with induction of cell division in quiescent, non-dividing cells. In those studies we selected mutant cell lines unable to divide in response to the polypeptide growth factor epidermal growth factor (EGF) (Pruss and Herschman, 1977; Terwilliger and Herschman, 1984) or the mitogen/tumor promoter tetradecanoyl phorbol acetate (TPA) (Butler-Gralla and Herschman, 1981). We isolated a number of independently derived 3T3 murine fibroblast variant cell lines unable to mount a mitogenic response either to EGF or to TPA and characterized the biochemical and physiological deficits in the EGF nonproliferative or the TPA nonproliferative derivatives. At the same time that we were isolating and characterizing mitogen nonproliferative variant lines, we also initiated a molecular program designed to identify genes induced in response to the mitogen/tumor promoter TPA. Our studies with the cDNAs cloned as a result of our search for TPA-induced gene expression have led us to a consideration of these genes, in response to a number of biological response modifiers, in cells of nervous system origin. In this chapter I will describe our isolation of these cDNAs, and their expression in several in vitro preparations derived from the nervous system.

Keywords

PC12 Cell Nerve Growth Factor Fibroblast Growth Factor Mitogenic Response Biological Response Modifier 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Harvey R. Herschman
    • 1
  1. 1.Department of Biological Chemistry, and Laboratory of Biomedical and Environmental SciencesUCLA Center for the Health SciencesLos AngelesUSA

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