Structure-Function Relationships for High and Low-Affinity Interleukin 2 Receptors
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The original study of interleukin 2 (IL-2) binding to activated lymphocytes by Robb and colleagues1 described a high-affinity receptor-ligand association which fit nicely with the very low levels of IL-2 which promoted cellular proliferation. Later studies, however, demonstrated that the IL-2 receptor actually existed in a number of forms with drastically different affinities for ligand2. Our subsequent research has focused on explaining these differences in molecular terms and relating them to the function of this versatile growth and differentiation factor. Insight into structure-function relationships for the IL-2-receptor system should prove particularly valuable to the design of drugs which can be used to manipulate IL-2-dependent immunological responses.
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- 2.R. J. Robb, W. C. Greene, and C. M. Rusk, Low and high affinity cellular receptors for interleukin 2: implications for the level of Tac antigen, J. Exp. Med. 16Ó: 1126 (1984).Google Scholar
- 3.R. J. Robb, C. M. Rusk, J. Yodoi, and W. C. Greene, An interleukin 2 binding molecule distinct from the Tac protein: analysis of its role in formation of high-affinity receptors, Proc. Natl. Acad. Sci. USA 84: 2002 (1987).Google Scholar
- 14.M. P. Neeper, L.-M Kuo, M. C. Kiefer, and R. J. Robb, Structure-function relationships for the IL 2-receptor system. III. Tac protein missing amino acids 102–173 (exon 4) is unable to bind IL 2. Detection of spliced protein after L cell transfection, J. Immunol. 138: 3532 (1987).PubMedGoogle Scholar
- 15.C. M. Rusk, M. P. Neeper, L.-M. Kuo, R. M. Kutny and R. J. Robb, Structure-function relationships for the IL-2 receptor system. V. Structure-activity analysis of modified and truncated forms of the Tac receptor protein. Site-specific mutagenesis of cysteine residues, J. Immunol. in press.Google Scholar
- 16.J. R. Ortaldo, A. T. Mason, J. P. Gerard, L. E. Henderson, W. Farrar, R. F. Hopkins, R. B. Herberman, and H. Rabin, Effects of natural and recombinant IL-2 on regulation of IFN 3d production and natural killer activity: lack of involvement of the Tac antigen for these immunoregulatory effects, J. Immunol. 133: 779 (1984).PubMedGoogle Scholar