Changes in Early and Late Steps of T Cell Activation by WT31 MoAb
The induction of T cell activation is usually a consequence of interaction between specific T cell receptors and their ligands. The T cell antigen receptor (TcR) is present on almost all T cells as a heterodimer of α and β glycoprotein (reviewed in 1). The TcR heterodimer is non-covalently associated with CD3 glycoprotein forming a TcR/CD3 complex (1). The TcR is responsible for antigen recognition and the CD3 antigen for signal transduction (reviewed in 2). Recently a monoclonal antibody (MoAb), WT31, has been described that recognizes a framework determinant present on all mature T lymphocytes that express α/β TcR heterodimer (3). In this investigation we have examined the effect of WT31 MoAb on some of the early and late steps of T cell activation to determine whether the WT31 moAb will induce signal transduction and activate human T cells. We show that WT31 activates human T cells via a classical signal transduction pathway.
KeywordsPhorbol Myristate Acetate Phorbol Myristate Acetate Induce Signal Transduction Cytoplasmic Free Calcium Autologous Mixed Lymphocyte Reaction
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