Effects of Bone Marrow Ablation on Compartmental Prostaglandin Synthesis by Mononuclear Phagocytes

  • A. Volkman
  • Y. Shibata
  • W. Dempsey
  • P. S. Morahan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 239)


It is well known that comparisons of populations or compartments of mononuclear phagocytes show considerable diversity of cellular function. In the case of resident-type macrophages (M⌽), such differences are widely believed to result from influences characteristic of individual compartments exerted on less specialized immigrant monocytes following their ongoing influx from the blood into tissues and tissue spaces. In this connection, data supporting the compartmental regulation of M⌽ arachidonic acid metabolism, at least in the pleural and the peritoneal cavities, were recently reported (1). The data we present show that in the spleen, M⌽ arachidonic acid metabolism is in part an expression of regulatory mechanisms at a distant site, the bone marrow. This demonstration required a model of bone marrow ablation and monocyte depletion, the principles of which are described below.


Bone Marrow Blood Monocyte Mononuclear Phagocyte Arachidonic Acid Metabolism Calcium Ionophore A23187 
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  1. 1.
    Fels, A., N. Pawlowski, E. Abraham and Z. Cohn. 1986. Compartmentalized regulation of macrophage arachidonic acid metabolism. J. Exp. Med. 163: 752–757.Google Scholar
  2. 2.
    van Furth, R., Z.A. Cohn, J.G. Hirsch, J.H. Humphrey, W.G. Spector and H.L. Langevoort. 1972. The mononuclear phagocyte system: a new classification of macrophages, monocytes and their precursor cells. Bull. WHO 46: 845.Google Scholar
  3. 3.
    van Furth, R., L. Diesselhoff and M. Dulk. 1984. Dual origin of mouse spleen macrophages. J. Exp. Med. 160: 1273.Google Scholar
  4. 4.
    Volkman, A., and J.L. Gowans. 1965. The production of macrophages in the rat. Br. J. Exp. Path. 50.Google Scholar
  5. 5.
    Volkman, A., and J.L. Gowans. 1965. The origin of macrophages from bone marrow in the rat. Br. J. Exp. Path. 46: 62.Google Scholar
  6. 6.
    Thompson, J., and R. van Furth. 1970. The effect of glucocorticosteroids on the kinetics of mononuclear phagocytes. J. Exp. Med. 131: 429.Google Scholar
  7. 7.
    Raper, J.R. 1964. Beta rays: biologic effects, in: “Medical Physics II,” O. Glasser, ed., p. 66, Chicago, The Year Book Publishers, Inc.Google Scholar
  8. 8.
    Sawyer, R.T., P.H. Strausbauch and A. Volkman. 1982. Resident macrophage proliferation in mice depleted of blood monocytes by strontium-89. Lab. Invest. 46: 165.Google Scholar
  9. 9.
    Morahan, P.S., W.L. Dempsey, A. Volkman and J. Connor. 1986. Antimicrobicidal activity of various immunomodulators: independence from normal levels of circulating monocytes and natural killer cells. Infect. Immun. 51: 87.Google Scholar
  10. 10.
    Bennett, M., and V. Kumar. 1983. 89Sr-induced bone marrow aplasia: effects on seed (stem cells) and soil (inductive microenvironment). Lab. Invest. 49: 235.Google Scholar
  11. 11.
    van Furth, R., and W. Sluiter. 1985. Macrophages as autoregulators of mononuclear phagocyte proliferation, in: “Macrophage Biology,” R. Sand and M. Kojuma, ed., p. 111–123, Alan R. Liss, New York.Google Scholar
  12. 12.
    Volkman, A., N.C. Chang, P.H. Strausbauch and P.S. Morahan. 1983. Differential effects of chronic monocyte depletion on macrophage populations. Lab. Invest. 49: 291.Google Scholar
  13. 13.
    Goodman, J.W. 1963. Transplantation of peritoneal fluid cells. Transplantation 3: 334–346.CrossRefGoogle Scholar
  14. 14.
    DeBakker, J.M., A.W. deWit, J.J.M. Onderwater, L.A. Ginsel and W.T. Daems. 1985. On the origin of peritoneal resident macrophages. II. Recovery of the resident macrophage population in the peritoneal cavity and in the milky spots after peritoneal cell depletion. J. Submicroscop. Cytol. 17: 141.Google Scholar
  15. 15.
    Shibata, Y., and A. Volkman. 1985. The effect of bone marrow depletion on prostaglandin E-producing suppressor macrophages in mouse spleen. J. Immunol. 135: 3897–3904.PubMedGoogle Scholar
  16. 16.
    Humes, J.L., S. Burger, M. Galavage, F.A. Kuehl, Jr., P.D. Wightman, M.E. Dahlgren, P. Davies and R.J. Bonney. 1980. The diminished production of arachidonic acid oxygenation products by elicited mouse peritoneal macrophages: possible mechanisms. J. Immunol. 124: 2110–2116.Google Scholar
  17. 17.
    Shibata, Y., A.P. Bautista, S.N. Pennington, J.L. Humes and A. Volkman. 1986. Eicosanoid production by peritoneal and splenic macrophages in mice depleted of bone marrow by 89Sr. Am J. Path., accepted for publication.Google Scholar
  18. 18.
    Rouzer, C.A., W.A. Scott, A.L. Hamill and Z.A. Cohn. 1982. Synthesis of leukotriene C and other arachidonic acid metabolites by mouse pulmonary macrophages. J. Exp. Med. 155: 720–733.Google Scholar
  19. 19.
    Shibata, Y., and A. Volkman. 1985. The effect of hemopoietic microenvironment on splenic suppressor macrophages in congenitally anemic mice of genotype Sl/Sld. J. Immunol. 135: 3905–3910.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • A. Volkman
    • 1
  • Y. Shibata
    • 1
  • W. Dempsey
    • 2
  • P. S. Morahan
    • 2
  1. 1.Department of PathologyEast Carolina University School of MedicineGreenvilleUSA
  2. 2.Department of Microbiology and ImmunologyMedical College of PennsylvaniaPhiladelphiaUSA

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