Use of Tissue Cultures as Model Systems for the Study of Lipid Biochemistry in Olives

  • Mark Williams
  • John Harwood

Abstract

Plant tissue technology was established, more or less, by Haberladt (see Yeoman & Aitchinson 1973) at the beginning of the twentieth century. He attempted to capitalize on the regenerative ability of higher plants in order to reproduce complete plants. The next major advancement was almost four decades later when callus tissue from tobacco and carrot was cultured successfully for extended periods (see Dodds & Roberts 1985). From these modest beginnings, plant tissue technology has developed to its present state. In fact, the term tissue culture actually might be a misnomer because, under in vitro conditions, cells often grow as an amorphous mass and exhibit little or no tissue morphology. With the discovery of kinetin (Miller et al. 1955) and the subsequent classic experiments by Skoog and Miller (1957), the role of plant growth regulators in plant growth and development has been clearly elucidated. Since then, the use of such plant growth regulators has greatly facilitated the maintenance of cells in culture while, at the same time, preserving their totipotency.

Keywords

Lipid Class Callus Culture Olive Fruit Olive Cultivar Olea Europaea 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Canas, L. A. (1988). In vitro culture of the olive tree (Olea europaea L.): present aspects and prospects. Bull Soc Bot Fr 135, Lettres bot 3,263–277.Google Scholar
  2. Canas, L. A., Wyssmann, A. M. & Benbadis, M. C. (1987). Isolation, culture and division of olive (Olea europaea L.) proplastids. Plant Cell Rep 6, 369–371.CrossRefGoogle Scholar
  3. Cocking, E. C. (1974). Ultrastructure of cultured plant cells. In Dynamic Aspects of Plant Ultrastructure, pp. 310–311. Edited by A. W. Robarts. London: McGraw-Hill.Google Scholar
  4. Datko, A. H. & Mudd, S. H. (1988). Phosphatidylcholine synthesis. Differing patterns in soybean and carrot. Plant Physiol 88, 854–861.CrossRefGoogle Scholar
  5. Dodds, J. H. & Roberts, L. W. (1985). Experiments in Plant Tissue Culture. Cambridge: Cambridge University Press.Google Scholar
  6. Eccleston, V. S. & Harwood, J. L. (1995). Solubilisation, partial purification and properties of acylCoA: glycerol-3-phosphate acyltransferase from avocardo (Persea americana) fruit mesocarp. Biochim Biophys Acta 1257, 1–10.CrossRefGoogle Scholar
  7. Fell, D. (1997). Understanding the Control of Metabolism. London: Portland Press.Google Scholar
  8. Gunstone, E D., Harwood, J. L. & Padley, E B. (eds.) (1994). The Lipid Handbook, 2nd ed. London: Chapman & Hall.Google Scholar
  9. Harwood, J. L. (1980). Plant acyl lipids: structure, distribution and analysis. In The Biochemistry of Plants, vol. 4, pp. 1–55. Edited by P. K. Stumpf & E. E. Conn. New York: Academic Press. Google Scholar
  10. Harwood, J. L. (1989). Lipid metabolism in plants. Crit Rev Plant Sci 8, 1–43.CrossRefGoogle Scholar
  11. Hilditch, T. P. & Williams, P. N. (1964). The Chemical Constitution of Natural Fats. London: Chapman & Hall.Google Scholar
  12. Kacser, H. B. & Burns, J. A. (1973). The control of flux. Symp Soc Exp Biol 32, 65–104.Google Scholar
  13. Kates, M. & Marshall, M. O. (1975). Biosynthesis of phosphoglycerides in plants. In Recent Advances in the Chemistry and Biochemistry of Plant Lipids, pp. 115–159. Edited by T. Galliard & E. I. Mercer. London: Academic Press.Google Scholar
  14. Lentza-Rios, C. (1994). Monitoring pesticide residues in olive products-organophosphorus insecticide in olives and oil. JAOAC Int 77, 1096–1100.Google Scholar
  15. Miller, C. O., et al. (1955). Kinetin, a cell division factor from deoxyribonucleic acid. JAm Chem Soc 77, 1392.CrossRefGoogle Scholar
  16. Montedoro, G., Bertuccicli, M. & Anichini, E. (1978). Aroma analysis of virgin olive oil by head space(volatiles) and extraction (polyphenols) technique. In Flavor of Foods and Beverages: Chemistryand Technology, p. 247–281. Edited by G. Charalambous & G. E. Inglett. New York: Academic Press.Google Scholar
  17. Moore, T. S. (1982). Phospholipid biosynthesis. Ann Rev Plant Physiol 33, 235–259.CrossRefGoogle Scholar
  18. Morales, M. T., Aparicio, R. & Rios, J. J. (1994). Dynamic headspace gas chromatographic method for determining volatiles in virgin olive oil. J Chromatogr. A 668, 455–462.CrossRefGoogle Scholar
  19. Murashige, T. & Skoog, E (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473–497.CrossRefGoogle Scholar
  20. Murphy, D. J. (1994). Biogenesis, function and biotechnology of plant storage lipids. Prog Lipid Res 33, 71–85.CrossRefGoogle Scholar
  21. Ohlrogge, J. B. & Jaworski, J. G. (1997). Regulation of fatty acid synthesis. Ann Rev Plant Physiol Plant Mol Biol 48, 109–136.CrossRefGoogle Scholar
  22. Orinos, Th. & Mitrakos, K. (1991). Rhizogenesis and somatic embryogenesis in calli from wild olive (Olea europaea var. sylvestris [Miller] Lehr) mature zygote embryos. Plant Cell, Tissue Organ Culture 27, 183–187.CrossRefGoogle Scholar
  23. Page, R. A., Okada, S. & Harwood, J. L. (1994). Acetyl-CoA carboxylase exerts strong flux control over lipid synthesis in plants. Biochim Biophys Acta 1210, 369–372.CrossRefGoogle Scholar
  24. Perry, H. J. & Harwood, J. L. (1993). Radiolabelling studies of acyl lipids in developing seeds of Bras-sica napus: use of [1–14C]acetate precursor. Phytochemistry 33, 329–333.CrossRefGoogle Scholar
  25. Price-Jones, M. J. & Harwood, J. L. (1986). The control of CTP:choline-phosphate cytidylyltransferase activity in pea (Pisum sativum L.). Biochem J 240, 837–842.Google Scholar
  26. Radwan, S. S. & Mangold, H. K. (1980). Biochemistry of lipids in plant cell cultures. In Advances inBiochemical Engineering, vol. 16, pp. 109–133. Edited by A. Fleichter. Berlin: Springer-Verlag.Google Scholar
  27. Ramli, U. S., Quant, P. A. & Harwood, J. L. (1998). Biochemical studies of oil synthesis in olive (Oleaeuropaea) and oil palm (Elaeis guineesis) callus cultures. Biochem Soc Trans 26, 151.Google Scholar
  28. Rapoport, T. A., Heinrich, R., Jacobasch G. & Rapoport, S. (1974). A linear steady-state treatment of enzymatic chains. EurJBiochem 42, 107–120.CrossRefGoogle Scholar
  29. Roughan, P. G. & Slack, C. R. (1982). Cellular organisation of glycerolipid metabolism. Ann Rev Plant Physiol 33, 97–132.CrossRefGoogle Scholar
  30. Rugini, E. (1986). Olive (Olea europaea L.). In Biotechnology in Agriculture and Forestry, vol. 1, pp. 253–266. Edited by P. S. Bajaj. Berlin: Springer-Verlag.Google Scholar
  31. Rutter, A. J. (1994). Lipid biosynthesis in olives. Ph.D. thesis, University of Wales.Google Scholar
  32. Rutter, A. J., Sanchez, J. & Harwood, J. L. (1997). Glycerolipid synthesis by microsomal fractions from Olea europaea fruits and tissue cultures. Phytochemistry 46, 855–862.CrossRefGoogle Scholar
  33. Rutter, A. J., Sanchez, J. & Harwood, J. L. (1998). The effect of dimethoate on lipid biosynthesis in olive (Olea europaea) callus cultures. Phytochemistry 47, 735–741.CrossRefGoogle Scholar
  34. Seidow, J. N. (1991). Plant lipoxygenase: structure and function. Ann Rev Plant Physiol Plant Mol Bio l 42, 145–188.Google Scholar
  35. Skoog, F. & Miller, C. O. (1957). Chemical regulation of growth and organ formation in plant tissue cultures in vitro. Symp Soc Exptl Biol 11, 118–140.Google Scholar
  36. Stymne, S. & Stobart, A. K. (1987). Triacylglycerol biosynthesis. In The Biochemistry of Plants, vol. 9, pp. 175–214. Edited by P. K. Stumpf & E. E. Conn. New York: Academic Press.Google Scholar
  37. Tran Thanh Van, K. M. (1981). Control of morphogenesis in in vitro cultures. Ann Rev Plant Physiol 32, 291–311.CrossRefGoogle Scholar
  38. de la Vega, M., Harwood, J. L. & Sanchez, J. (1992). Effect of dimethoate on lipid metabolism in olive fruit. In Metabolism, Structure and Utilisation of Plant Lipids, pp. 368–371. Edited by A. Cherif et al. Tunis: Centre Pedagogique.Google Scholar
  39. Vigh, L., et al. (1993). The primary signal in the biological perception of temperature: Pd-catalysed hydrogenation of membrane lipids stimulated the expression of the desA gene in Synechocystis PCC6803. Proc Natl Acad Sci, USA 90, 9090–9094.CrossRefGoogle Scholar
  40. Wang, K., et al. (1979). Cytohistological studies in the tissue culture of Olea europaea L. II. Histogenesis and organogenesis. Acta Botanica Sinica 21, 225–236.Google Scholar
  41. Wharfe, J. & Harwood, J. L. (1978). Fatty acid biosynthesis in the leaves of barley, wheat and pea. Biochem J 174, 163–169.Google Scholar
  42. Williams, M. (1992). Lipid metabolism in tissue cultures of Brassica napus L. and Olea europaea L. Ph.D. thesis, University of Wales.Google Scholar
  43. Williams, M. & Harwood, J. L. (1994). Alternative pathways for phosphatidylcholine synthesis in olive (Olea europaea L.) callus cultures. Biochem J 304, 463–468.Google Scholar
  44. Williams, M. & Harwood, J. L. (1998). Reaction products of the lipoxygenase pathway in olive tissue cultures. Biochem Soc Trans 26, 154.Google Scholar
  45. Williams, M., et al. (1993). Lipid biosynthesis in olive cultures. JExptl Bot 44, 1717–1723.CrossRefGoogle Scholar
  46. Williams, M., et al. (1998). Analysis of volatiles from callus cultures of olive Olea europaea. Phytochemistry 47, 1253–1259.CrossRefGoogle Scholar
  47. Yeoman, M. M. & Aitchinson, P. A. (1973). Growth patterns in tissue (callus) cultures. In Plant Tissue and Cell Culture,pp. 240–268. Edited by H. E. Street. Oxford: Blackwell Scientific Publications.Google Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Mark Williams
  • John Harwood

There are no affiliations available

Personalised recommendations