Use of Tissue Cultures as Model Systems for the Study of Lipid Biochemistry in Olives

  • Mark Williams
  • John Harwood


Plant tissue technology was established, more or less, by Haberladt (see Yeoman & Aitchinson 1973) at the beginning of the twentieth century. He attempted to capitalize on the regenerative ability of higher plants in order to reproduce complete plants. The next major advancement was almost four decades later when callus tissue from tobacco and carrot was cultured successfully for extended periods (see Dodds & Roberts 1985). From these modest beginnings, plant tissue technology has developed to its present state. In fact, the term tissue culture actually might be a misnomer because, under in vitro conditions, cells often grow as an amorphous mass and exhibit little or no tissue morphology. With the discovery of kinetin (Miller et al. 1955) and the subsequent classic experiments by Skoog and Miller (1957), the role of plant growth regulators in plant growth and development has been clearly elucidated. Since then, the use of such plant growth regulators has greatly facilitated the maintenance of cells in culture while, at the same time, preserving their totipotency.


Lipid Class Callus Culture Olive Fruit Olive Cultivar Olea Europaea 
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© Springer Science+Business Media New York 2000

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  • Mark Williams
  • John Harwood

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