Abstract
No other component of the microscope is as instrumental in determining the information content of an image as the objective. The resolved detail, the contrast at which this detail is presented, the depth through the object from which useful information can be derived and the diameter of the useful field are all limited by the performance of the objective. All other imaging components, such as relay optics, Telan systems, tube lenses and eyepieces, or pro-jectives may have some corrective function but otherwise serve only to present the image generated by the objective to the detector in such a way that most of its information content can be recorded without degradation.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Inoué, S., and Oldenbourg, R., 1993, Optical instruments: Microscopes. In: Handbook of Optics (Optical Society of America, ed), 2nded. McGraw-Hill, New York.
Maly, M., and Boy de, A., 1994, Real-time steroscopic confocal reflection confocal microscopy using objective lenses with linear longitudinal chromatic dispersion, Scanning 16:187–193.
Pawley, J.B., Amos, W.B., Dixon, A., Brelje, T.C., 1993, Simultaneous, non-interfering collection of optimal fluorescent andbackscattered light signals on the MRC 500/600. In: Proceedings of the Fifty-first Annual Meeting of the Microscopy Society of America, San Francisco Press, Inc., San Francisco, pp. 156–157.
Petroll, W.M., Cavanaugh, H.D., and Jester, J.V., 1993, Three dimensional imaging of corneal cells using in vivo vconfocal microscopy, J. Microsc. 170:213–219.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Keller, H.E. (1995). Objective Lenses for Confocal Microscopy. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5348-6_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-5350-9
Online ISBN: 978-1-4757-5348-6
eBook Packages: Springer Book Archive