Imaging Immunogold Labels with Confocal Microscopy

  • Carol J. Cogswell

Abstract

During more than a decade of biological confocal microscopy, the feature that has generally received the most emphasis is its capacity to achieve improved axial resolution when imaging fluorescently labeled preparations. This single attribute has resulted in a continuing codevelopment of confocal imaging systems together with new fluorescent dyes designed specifically to exploit the improved spatial and temporal imaging provided by these microscopes. Although fluorescence applications are continuing to receive considerable attention, we are also beginning to explore other optical modes of the confocal microscope to image, in three dimensions (3D), the enormous range of biological preparations that can be labeled with nonfluorescing probes.

Keywords

Colloidal Gold Green Laser Axial Resolution Chromatic Aberration Blue Laser 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Carol J. Cogswell
    • 1
  1. 1.Physical Optics Department, School of PhysicsUniversity of SydneySydneyAustralia

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