Abstract
Optical microscopy has always been a central technique to biological research, but in recent years its importance has vastly increased, mainly because of the introduction of epifluorescence imaging, which gives very sensitive detection, coupled with a multitude of highly specific fluorescent probes. Furthermore, it is often possible to obtain useful images noninvasively at light levels that are not damaging to living cells. Microinjection and other cell-loading methods can therefore be used in combination with fluorescence microscopy to analyze and modify subcellular structure and function in living cells.
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References
Agard, D. A., and Sedat, J.W., 1983, Three-dimensional architecture of apolytene nucleus, Nature 302:676–681.
Agard, D.A., Hiraoka, Y., Shaw, P.J., and Sedat J.W., 1989, Fluorescence microscopy in three dimensions, Methods Cell Biol. 30:353–378.
Aikens, R.S., Agard, D.A., and Sedat, J.W., 1989, Solid state imagers for microscopy, Methods Cell Biol. 29:291–313.
Carrington, W.A., Fogarty, K.E., and Fay, F.S., 1990, 3D fluorescence imaging of single cells using image restoration. In: Non-invasive Techniques in Cell Biology (Foskett and Grinstein, eds.), A.R. Liss, New York.
Carter, K.C., Bowman, D., Carrington, W., Fogarty, K., McNeil, J.A., Fay, F.S., and Lawrence, J.B., 1993, A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus, Science 259:1330–1335.
Castleman, K.R., 1979, Digital Image Processing, Prentice-Hall, Englewood Cliffs, New Jersey.
Erhardt, A., Zinser, G., Komitowski, D., and Bille, J., 1985, Reconstructing 3D light microscopic images by digital image processing, Appl. Opt. 24:194.
Fay, F.S., Carrington, W., andFogarty, K.E., 1989, Three-dimensional molecular distribution in single cells analyzed using a digital imaging microscope, J. Microsc, 153 (pt 2): 133–149.
Flanders, D.J., Rawlins, D.J., Shaw, P.J., and Lloyd, C.W., 1990, Re-establishment of the interphase microtubule array in vacuolated plant cells, studied by confocal microscopy and 3-D imaging, Development 110:897–904.
Frieden, B.R., 1984, Maximum-likelihood estimates of spectra. In: Deconvolu-tion with Applications in Spectroscopy (P.A. Jansson, ed), Academic Press, New York, pp. 229–261.
Highett, M.I., Rawlins, D.J., and Shaw, P.J., 1993a, Different patterns of rDNA distribution in Pisum sativum nucleoli correlate with different levels of nucleolar activity, J. Cell Sci. 104:843–852.
Highett, M.I., Beven, A.F., and Shaw, P.J., 1993b, Localization of 5S genes and transcripts in Pisum sativum nuclei, J. Cell Sci. 105:1151–1158.
Hiraoka, Y., Sedat, J.W., and Agard, D.A., 1988, The use of a charge-coupled device for quantitative optical microscopy of biological structures, Science 238:36–41.
Hiraoka, Y., Minden, J.S., Swedlow, J.R., Sedat, J.W., and Agard, D.A., 1989, Focal points for chromosome condensation and decondensation from three-dimensional in vivo time-lapse microscopy, Nature 342:293–296.
Hiraoka, Y., Sedat, J.W., and Agard, D.A., 1990, Determination of three-dimensional imaging properties of a light microscope system: Partial confocal behaviour in epifluorescence microscopy, Biophys. J. 57:325–333.
Holmes, T.J., 1988, Maximum-likelihood restoration adapted for noncoherent optical imaging, J. Opt. Soc. Am. A5:666–673.
Holmes, T.J., and Liu, Y.-H., 1992, Image restoration for 2-D and 3-D fluorescence microscopy. In: Visualization in Biomedical Microscopies (A. Kriete, ed), VCH, Weinheim, Germany.
Inoué, S., 1986, Video Microscopy, Plenum Press, New York.
Jansson, P.A., Hunt, R.M., and Plyler, E.K., 1970, J. Opt. Soc. Am. 60:596.
Pawley, J.B., 1994, The sources of noise in three-dimensional microscopical data sets. In: Three-dimensional Confocal Microscopy: Volume Investigation of Biological Specimens (J.K. Stevens, L.R. Mills, and J.E. Trogadis, eds.), Academic Press, New York, pp. 48–94.
Pawley, J.B., and Smallcomb, A., 1992, An introduction to practical confocal microscopy: The ultimate form of biological light microscopy? Acta Microsc. 1:58–73.
Sandison, D.R., Piston, D.W., and Webb, W.W., 1994, Background rejection and optimization of signal-to-noise in confocal microscopy. In: Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens (J.K Stevens, L.R. Mills, and J. E Trogadis., eds), Academic Press, New York, pp. 29–47.
Self, S.A., 1983, Focusing of spherical Gaussian beams, Appl. Opt. 22:658–661.
Shaw, P.J., 1993, Computer reconstruction in three-dimensional fluorescence microscopy. In: Electronic Light Microscopy (D. Shotton, ed), Wiley-Liss, New York, pp. 211–230.
Shaw, P.J., and Rawlins, D.J., 1991a, Three-dimensional fluorescence microscopy, Prog. Biophys. Molec. Biol. 56:187–213.
Shaw, P.J., and Rawlins, D.J., 1991b, The point spread function of a confocal microscope: Its measurement and use in deconvolution of 3D data, J. Microsc. 163:151–165.
Sheppard, C.J.R., and Choudhury, A., 1977, Image formation in the scanning microscope, Opt. Acta. 24:1051–1073.
Stokseth, P.A., 1969, Properties of a defocused optical system, J. Opt. Soc. Am. 59:1314–1321.
Wilson, T., 1993, Image formation in confocal microscopy. In: Electronic Light Microscopy (D.M. Shotton, ed), Wiley-Liss, New York.
Young, I.T., 1989, Image fidelity: Characterizing the imaging transfer function, Methods Cell Biol. 30:2–47.
Zhang, D.H., Wadsworth, P., and Hepler, P.K., 1990, Microtubule dynamics in living dividing plant cells: Confocal imaging of microinjected fluorescent brain tubulin, Proc. Natl Acad. Sci. USA. 87:8820–8824.
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Shaw, P.J. (1995). Comparison of Wide-Field/Deconvolution and Confocal Microscopy for 3D Imaging. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_23
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DOI: https://doi.org/10.1007/978-1-4757-5348-6_23
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