Real-Time Stereo (3D) Confocal Microscopy
Three-dimensional (3D) microscopy requires the collection of data over a certain volume in the specimen, followed by a suitable two-dimensional (2D) visualization procedure in which the desired image is produced. In holography, nature offers us a potential pathway by which the 3D structure of an object can be observed directly through wavefront reconstruction techniques. However, the slow time response of holographic recording media prevents real-time applications of this technique. In addition, while in principle this approach might work in reflection, holographic fluorescence images are impossible because emitted fluorescence radiation is incoherent.
KeywordsConfocal Image Emission Flux Emission Capability Readout Noise Camera Plane
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