Lens Aberrations in Confocal Fluorescence Microscopy
Modern optical microscopes are so good that many scientists forget that these instruments only provide their optimal performance if they are used under certain operating conditions. Typical users may be unaware of the very existence of such limitations because they may unwittingly work within the limits or fail to recognize their effects. It is probably also correct to assume that the engineers who designed the instrument did not expect the scientist to use it with devices that exceed the sensitivity and intra-scene dynamic range of the human eye or photographic film. Last but not least, the manufacturer does not intend to discourage purchase by emphasizing the limits imposed by the specifications of the instrument.
KeywordsObjective Lens Numerical Aperture Mount Medium Axial Resolution Focal Shift
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