Image Contrast in Confocal Light Microscopy

  • P. C. Cheng
  • A. Kriete


In any form of microscopy, one needs not only an imaging system with enough resolution to reveal the fine details of a specimen but also a suitable contrast mechanism by which to “see” the structures of interest. As defined by the New Webster ′s Dictionary, contrast is the difference between light and dark areas of a negative or print. In other words, contrast is the difference in signal strength between various parts of an image or between details of interest and “background.” In science, the contrast is proportional to the intensity difference (ΔI) between two image areas, divided by the average image brightness Ī.


Image Contrast Objective Lens Volume Rendering Optical Section Methyl Salicylate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • P. C. Cheng
    • 1
  • A. Kriete
    • 2
  1. 1.Advanced Microscopy and Imaging Laboratory, Department of Electrical and Computer Engineering and Department of Biological SciencesState University of New York at BuffaloBuffaloUSA
  2. 2.Institute for Anatomy and CytobiologyUniversity of GiessenGiessenGermany

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