Intermediate Optics in Nipkow Disk Microscopes

  • G. S. Kino


The confocal laser-scanning optical microscope (CLSM) has the major advantages ofyielding a very short depth of focus and of being very well suited to optical cross-sectioning. Its transverse resolution, dxy, and the contrast of the image are better than with a standard microscope, and because it uses a laser beam, the illumination intensity is very high. At the same time, a choice of illumination wavelengths is available (Wilson and Sheppard, 1984).


Tube Length Standard Microscope Pupil Plane Transverse Resolution Pinhole Size 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • G. S. Kino
    • 1
  1. 1.Edward L. Ginzton LaboratoryStanford UniversityStanfordCaliforniaUSA

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