Membrane Aging of the Brain Synaptosomes with Special Reference to Gangliosides
Giacobini (1982) has proposed an idea of synaptic aging, according to which synapses are functionally developed to the maximal state in the developmental stage, and then may deteriorate in their function in senescence. Giacobini emphasized the relevance of reduced synaptic transmission to malfunction of the aging brain. Agerelated alterations of synaptic functions have been reported in terms of the levels, synthetic rates and releasing activities of neurotransmitters. Gibson and Peterson (1981a) reported the reduced synthetic rate of acetylcholine in aged mice with little change in acetylcholine content. Gibson and Peterson (1981b) also pointed out that the release of acetylcholine is severely affected with aging. We assumed that possible alterations of synaptic membranes would be responsible for the decrease In neurotransmission. We, therefore, have isolated synaptosomes from different age groups of mice and rats, and attempted to correlate the membrane changes with altered synaptic functions such as acetylcholine release.
KeywordsFluorescence Polarization Acetylcholine Release Neuraminidase Treatment Total Ganglioside Membrane Microviscosity
thin layer chromatography
electron spin resonace.
Unable to display preview. Download preview PDF.