Gangliosides as Differential Modulators of Membrane-Bound Protein Kinase Systems

  • Robert K. Yu
  • James R. Goldenring
  • John Y. H. Kim
  • Robert J. DeLorenzo
Part of the FIDIA Research Series book series (FIDIA, volume 6)

Abstract

Gangliosides are sialic acid-containing glycosphingolipids found in the plasma membrane of virtually all vertebrate tissues and are particularly abundant in the nervous system (Ledeen and Yu, 1982; Ledeen, 1983; Ando, 1983; Ledeen, 1985). Gangliosides comprise a major part of the glycoconjugate network extending from the neuronal membrane surface. Recent investigations suggest that gangliosides may exert a critical role in the regulation of cell growth and differentiation (Hakomori, 1981; 1983; Ledeen et al., 1984; Ando, 1983; Ledeen, 1984). Nevertheless, the molecular events which underlie the physiological and cellular events remain unresolved. Recent investigations have emphasized the importance of Ca++-dependent kinase systems in the regulation of many dynamic cellular processes (Nestler et al., 1984; Cheung, 1982; DeLorenzo and Goldenring, 1984). In particular, the Ca+ +/calmodulin-dependent kinase (Kennedy, 1983) and the Ca+ + /phospholipid-dependent kinase (C-kinase) system (Nishizuka, 1984a,b) have been implicated as major regulators of cellular physiology. Gangliosides bind Ca+ + with high affinity (Abramson et al., 1972; Probst et al., 1979; 1984; Quarles and Folch-Pi, 1965; Rahmann, 1983), and thus, their possible interaction with Ca+ + -dependent kinases is of great interest. We present here our recent investigations which indicate that gangliosides may exert opposite effects on these Ca+ +-dependent kinases with inhibition of C-kinase and stimulation of calmodulin-dependent kinase (Goldenring et al., 1985; Kim et al., 1986).

Keywords

Myelin Basic Protein Brain Membrane High Performance Thin Layer Chromatography Dalton Protein Phosphocellulose Paper 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

EGTA

ethylene -bis(oxyethylenenitrile) tetraacetic acid

MBP

myelin basic protein

CaM

calmodulin

IC50

concentration for 50% inhibition

SDS

sodium dodecylsulphate

PAGE

polyacrylamide gel electrophoresis.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Robert K. Yu
    • 1
  • James R. Goldenring
    • 1
    • 2
  • John Y. H. Kim
    • 1
  • Robert J. DeLorenzo
    • 1
    • 3
  1. 1.Department of NeurologyYale University School of MedicineNew HavenUSA
  2. 2.Surgical Service (112)West Haven VA Medical CenterWest HavenUSA
  3. 3.Department of NeurologyMedical College of VirginiaRichmondUSA

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