Molecular Specificity of Ganglioside Action on Neurite Regeneration in Cell Cultures of Sensory Neurons

  • Patrick Doherty
  • John G. Dickson
  • Thomas P. Flanigan
  • Frank S. Walsh
Part of the FIDIA Research Series book series (FIDIA, volume 6)

Abstract

Studies of the antigenic sites recognised by the monoclonal antibody (McAb) A2B5 (Eisenbarth et al., 1979) and on the binding receptors for tetanus toxin (Van Heyningen, 1963; Mirsky et al., 1978) have demonstrated that neurons can express a relatively unique family of higher order gangliosides at their cell surface. The high abundance of gangliosides in nervous tissue (Wiegandt, 1971; Ledeen, 1978) considered together with the qualitative and quantitative changes apparent in the total ganglioside pool during the development of several brain areas (Kasai and Yu, 1983; Hilbig et al., 1984) lends support to the concept that endogenous gangliosides may somehow function in the process of neuritogenesis in the embryo and/or neurite regeneration in the adult. Supportive evidence for such a role comes from the observation that a complex mixture of bovine brain gangliosides can stimulate motor neurone sprouting in vivo (Gorio et al., 1983).

Keywords

Dorsal Root Ganglion Sensory Neuron Dorsal Root Ganglion Neuron Neuronal Cell Survival United Kingdom Introduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

NGF

nerve growth factor

DRG

dorsal root ganglia

McAb

monoclonal antibody

HRP-RAM

horse radish peroxidase-conjugated rabbit antimouse Ig

DMEM

Dulbecco’s modified Eagle’s medium

FCS

fetal calf serum

PBS

phosphate-buffered saline

HS

horse serum

OPD

orthophenylenediamine.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderton BH, Breinburg D, Downes MJ, Green PJ, Tomlison BE, Ulrich J, Wood JN, Kahn J (1982) Nature 298: 84 - 86.PubMedCrossRefGoogle Scholar
  2. Barde Y-A, Edgar D, Thoenen H (1980) Proc Natl Acad Sci USA 77: 1199 - 1203.PubMedCrossRefGoogle Scholar
  3. Bremer EG, Hakomori S, Bowen-Pope DF, Raines E, Ross R (1984) J Biol Chem 259: 6818 - 6825.PubMedGoogle Scholar
  4. Dickson JB, Flanigan TP, Walsh FS (1982) in: Rowland LP (ed): Human Motor Neuron Diseases. Raven Press, New York, pp. 435 - 451.Google Scholar
  5. Doherty P, Dickson JG, Flanigan TP, Walsh FS (1984a) J Neurochem 42: 1116 - 1122.PubMedCrossRefGoogle Scholar
  6. Doherty P, Dickson JG, Flanigan TP, Walsh FS (1984b) Neurosci Lett 51: 55 - 60.PubMedCrossRefGoogle Scholar
  7. Doherty P, Dickson JG, Flanigan TP, Walsh FS (1985) J Physiol 360: 43.P.Google Scholar
  8. Eisenbarth GS, Walsh FS, Nirenberg M (1979) Proc Natl Acad Sci USA 76: 4913 - 4917.PubMedCrossRefGoogle Scholar
  9. Facci L, Leon A, Toffano G, Sonnino S, Ghidoni R, Tettamanti G (1984) J Neurochem 42: 299 - 305.PubMedCrossRefGoogle Scholar
  10. Ferrari G, Fabris M, Gorio A (1983) Dev Brain Res 8: 215 - 221.CrossRefGoogle Scholar
  11. Gorio A, Marini P, Zanoni R (1983) Neuroscience 8: 417 - 429.PubMedCrossRefGoogle Scholar
  12. Van Heynigen WE (1963) J Gen Microbiol 31: 375 - 387.CrossRefGoogle Scholar
  13. Van Heyningen WE, Carpenter CCJ, Pierce NF, Greenough WB (1971) J Infect Dis 124: 415 - 418.PubMedCrossRefGoogle Scholar
  14. Hilbig, Lanke G, Rahman H (1984) Dev Neurosci 6: 260 - 270.CrossRefGoogle Scholar
  15. Kasai N and Yu RK (1983) Brain Res 277: 155 - 158.PubMedCrossRefGoogle Scholar
  16. Ledeen RW (1978) J Supramol Struct 8: 1 - 17.PubMedCrossRefGoogle Scholar
  17. Mirsky R, Wendon LMB, Black P, Stolkin C, Bray D (1978) Brain Res 148: 251 - 259.PubMedCrossRefGoogle Scholar
  18. Roisen FJ, Bartfeld H, Nagele L, Yorke G (1981) Science 214: 577 - 578.PubMedCrossRefGoogle Scholar
  19. Rybak S, Ginzburg I, Yavin E (1983) Biochem Biophys Res Commun 116: 974 - 980.PubMedCrossRefGoogle Scholar
  20. Schwartz M and Spirman N (1982) Proc Natl Acad Sci USA 79: 6080 - 6083.PubMedCrossRefGoogle Scholar
  21. Skaper, SD and Varon S (1985) Int J Dev Neurosci 3: 187 - 198.CrossRefGoogle Scholar
  22. Tettamanti G, Bonali F, Marchesini S, Zambotti V (1973) Biochim Biophys Acta 296: 160 - 170.PubMedCrossRefGoogle Scholar
  23. Toffano G, Benvegnù D, Bonetti AC, Facci L, Leon A, Orlando P, Ghidoni R, Tettamanti G (1980) J Neurochem 35: 861 - 866.PubMedCrossRefGoogle Scholar
  24. Toffano G, Savoini G, Moroni F, Lombardi G, Calza L, Agnati LF (1983) Brain Res 261: 163 - 168.PubMedCrossRefGoogle Scholar
  25. Wiegandt H (1971) Adv Lipid Res 9: 249 - 289.Google Scholar
  26. Wiegandt H (1976) Adv Exp Med Biol 71: 3 - 14.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Patrick Doherty
    • 1
  • John G. Dickson
    • 1
  • Thomas P. Flanigan
    • 1
  • Frank S. Walsh
    • 1
  1. 1.Institute of NeurologyQueen SquareLondonUK

Personalised recommendations