Evidence for the Effects of Gangliosides on the Development of Neurons in Primary Cultures
A useful tool to investigate the involvement of gangliosides in the molecular mechanisms leading to cellular differentiation and maturation is to culture cells in the presence of the glycolipids (Byrne et al., 1983; Fishman et al., 1983; Facci et al., 1984; Dreyfus et al., 1984a; Dreyfus et al., 1984b; Massarelli et al., 1985b) which are known to incorporate into the plasma membranes (Schwarzmann et al., 1985). In neurons, much experimental evidence has shown a neuronotrophic and a neuritogenic effect due to gangliosides (Byrne et al., 1983; Facci et al., 1984; Dreyfus et al., 1984b; Massarelli et al., 1985b; Ledeen, 1984). Previous experiments in our laboratory have shown that the addition of a mixture of gangliosides to the growth medium in primary cultures of chick brain neurons during the period of synaptogenesis led to stimulation of sprouting of the secondary neuronal processes (Dreyfus et al., 1984b; Massarelli et al., 1985a, 1985b). Furthermore, we were interested in the study of the effect of exogenous gangliosides added at seeding in neuronal cultures grown without serum. Some preliminary results have already been reported (Dreyfus et al., 1984c).
KeywordsAmine Content Neutral Glycolipid Total Ganglioside Exogenous Ganglioside Ganglioside Species
Dulbecco’s modified Eagle’s medium
Dulbecco’s modified Eagle’s medium with 20% fetal calf serum
modified Bottenstein-Sato defined medium
total neutral glycolipids
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