Abstract
Although peripheral neuropathy is a well-known complication of myeloma and other paraproteinemias, it has been uncertain whether these paraproteins play a primary role in the pathogenesis of neuropathy or not. Recently Braun et al. (1982) demonstrated that IgM monoclonal antibodies from patients with IgM paraproteinemia and polyneuropathy reacted with myelin-associated glycoprotein (MAG). Ilyas et al. (1984) reported that IgM from some of these patients reacted with both MAG and an acidic glycolipid in human peripheral nerve ganglioside fraction, and suggested that a carbohydrate determinant which reacted with these patients’ IgM was shared between MAG and this acidic glycolipid. We reported previously (Baba et al., 1985) a patient with IgM paraproteinemia and polyradiculoneuropathy whose serum only reacted with a lipid component in human peripheral nerve ganglioside fraction but not with MAG. We demonstrate here further studies on this glycolipid.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- MAG:
-
myelin associated glycoprotein
- SDS:
-
sodium dodecylsulphate
- PAP:
-
peroxidase antiperoxidase
- BSA:
-
bovine serum albumin
- DAB:
-
diaminobenzidine
- TLC:
-
thin layer chromatography
- SLPG:
-
sialosyllactosaminyl paragloboside.
References
Baba H, Miyatani N, Sato S, Yuasa T, Miyatake T (1985) Antibody to glycolipid in a patient with IgM paraproteinemia and polyradiculoneuropathy. Acta Neurol Scand 72: 218–221.
Braun PE, Frail DE, Latov N (1982) Myelin-associated glycoprotein is the antigen for a monoclonal IgM in polyneuropathy. J Neurochem 39: 1261–1265.
Chou KH, Ilyas AA, Evans JE, Quarles RH, Jungalwala FB (1985a) Structure of a glycolipid reacting with monoclonal IgM in neuropathy and with HNK-1. Biochem Biophys Res Commun 128: 383–388.
Chou KH, Nolan CE, Jungalwala FB (1985b) Subcellular fractionation of rat sciatic nerve and specific localization of ganglioside LM1 in rat nerve myelin. J Neurochem 44: 1898–1912.
Hays AP, Takatsu M, Latov N et al. (1983) Focal demyelination of cat sciatic nerve induced by intraneural injection of serum from patients with polyneuropathy and monoclonal IgM reactive with myelin-associated glycoprotein. J Neuropath Exp Neurol 42: 349.
Ilyas AA, Quarles RH, MacIntosh TD, Dobersen MJ, Trapp BD, Dalakas MC, Brady RO (1984) IgM in a human neuropathy related to paraproteinemia binds to a carbohydrate determinant in the myelin-associated glycoprotein and to a ganglioside. Proc Natl Acad Sci USA 81: 1225–1229.
Nakamura K, Nagashima M, Sekine M, Igarashi M, Ariga T, Atsumi T, Miyataka T, Suzuki A, Yamakawa T (1983a) Gangliosides of hog skeletal muscle. Biochim Biophys Acta 752: 291–300.
Nakamura K, Ariga T, Yahagi T, Miyatake T, Suzuki A, Yamakawa T (1983b) Interspecies comparison of muscle gangliosides by two-dimentional thin-layer chromatography. J Biochem (Tokyo) 94: 1359–1365.
Nemni,R, Galassi G, Latov N, Sherman WH, Olarte MR, Hays AP (1983) Polyneuropathy in nonmalignant IgM plasma cell dyscrasia: A morphological study. Ann Neurol 14: 43–54.
Norton WT and Poduslo SE (1973) Myelination in rat brain: Method of myelin isolation. J Neurochem 21: 749–757.
Sato S, Baba H, Tanaka M et al. (1983) Antigenic determinant shared between myelin-associated glycoprotein from human brain and natural killer cells. Biomed Res 4: 489–494.
Sherman WH, Latov N, Hays AP, Takatsu M, Nemni R, Galassi G, Osserman EF (1983) Monoclonal IgMk antibody precipitating with chondroitin sulfate C from patients with axonal polyneuropathy and epidermolysis. Neurology (NY) 33: 192–201.
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Baba, H., Miyatani, N., Sato, S., Nakamura, K., Yuasa, T., Miyatake, T. (1986). Antibody to Glycolipid in a Patient with IgM Paraproteinemia and Polyradiculoneuropathy. In: Tettamanti, G., Ledeen, R.W., Sandhoff, K., Nagai, Y., Toffano, G. (eds) Gangliosides and Neuronal Plasticity. FIDIA Research Series, vol 6. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-5309-7_13
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5309-7_13
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4757-5311-0
Online ISBN: 978-1-4757-5309-7
eBook Packages: Springer Book Archive