Folylpoly-γ-Glutamate Synthetases: Properties and Regulation

  • Barry Shane
  • David J. Cichowicz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 163)


The substrate specificities of folylpolyglutamate synthetases from Corynebacterium species, Lactobacillus casei, Streptococcus faecalis, and Chinese hamster ovary (CHO)** cells are described. The purified Corynebacterium enzyme catalyzes the addition of glutamate moieties to reduced pteroylmonoglutamates, and to 5,10-methylenetetra-hydropteroyldi- and triglutamates. The purified protein also possesses a separate dihydrofolate synthetase activity. Binding of pteroate substrates to dihydrofolate synthetase impairs folate binding to folylpolyglutamate synthetase. The in vivo distribution of folylpoly-glutamates in bacteria and mammalian cells, which differs from source to source, appears to be a reflection of the ability of folylpolyglut-amates to act as substrates for folylpolyglutamate synthetases from different sources. Bacterial synthetases use a variety of pteroylmonoglutamates as their preferred monoglutamate substrate, but use 5,10-methylenetetrahydropteroyl polyglutamates as their preferred, and sometimes only, polyglutamate substrates. Mono- and polyglutamyl forms of tetrahydrofolate are the preferred substrates of the CHO cell synthetase. Products of one carbon metabolism do not affect the in vivo distribution of folylpolyglutamates in CHO cells. Although adenine changes the distribution of folylpolyglutamates in bacteria, this effect is mediated by changing the substrate availability for the synthetase reaction, and not by regulating the levels of the synthetase per se.


Chinese Hamster Ovary Cell Chinese Hamster Ovary Synthetase Activity Lactobacillus Casei Standard Assay Mixture 
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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Barry Shane
    • 1
  • David J. Cichowicz
    • 1
  1. 1.Department of BiochemistryThe Johns Hopkins University School of Hygiene and Public HealthBaltimoreUSA

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