Stepwise Thermophotochemical Crosslinking for Enzyme Stabilization and Immobilization

  • P. Guire


Non-covalent bonding (e.g. electrostatic or ionic, hydrophobic and hydrogen bonding) is very important in maintaining the intra- and intermolecular interactions critical to the functioning of biological macromolecules in aqueous environments. The three-dimensional structure compatible with these functional interactions is stabilized in most enzymes by covalent non-peptide (e.g. disulfide) bonds. The introduction of additional covalent bonds within and between enzyme molecules thus seems to be a reasonable approach to the stabilization of the functional interactions for their subsequent study and use in unnatural environments.


Enzyme Molecule Carrier Material Crosslinking Reagent Acetyl Phosphate Dichlorophenol Indophenol 
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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • P. Guire
    • 1
  1. 1.Midwest Research InstituteKansas CityUSA

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