Stepwise Thermophotochemical Crosslinking for Enzyme Stabilization and Immobilization

  • P. Guire

Abstract

Non-covalent bonding (e.g. electrostatic or ionic, hydrophobic and hydrogen bonding) is very important in maintaining the intra- and intermolecular interactions critical to the functioning of biological macromolecules in aqueous environments. The three-dimensional structure compatible with these functional interactions is stabilized in most enzymes by covalent non-peptide (e.g. disulfide) bonds. The introduction of additional covalent bonds within and between enzyme molecules thus seems to be a reasonable approach to the stabilization of the functional interactions for their subsequent study and use in unnatural environments.

Keywords

Enzyme Molecule Carrier Material Crosslinking Reagent Acetyl Phosphate Dichlorophenol Indophenol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • P. Guire
    • 1
  1. 1.Midwest Research InstituteKansas CityUSA

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