Development of Immobilized Urease for the Owens-Illinois Bun Analyzer

  • M. H. Keyes
  • R. Barabino


The number of methods available to immobilize an enzyme has expanded in recent years until it is difficult to choose which method is best suited for a specific application (1). Yet the requirements of the support for any application as well as the charac-teristics of the enzyme can reduce considerably the number of methods which give satisfactory results. The development of immobilized urease for our BUN analyzer illustrates the process of selecting the optimum method for a specific application. The flow scheme of this analyzer is shown in Fig. 1. The sample of urea is injected with a calibrated microsyringe. After the sample passes through the urease cartridge, the ammonium ions generated are converted to ammonia in the mixer with sodium hydroxide solution. Finally, the ammonia is detected by an ammonia electrode.


Ceramic Disc Immobilize Urease Enzyme Derivative Porous Ceramic Material Insolubilizing Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    ZABORSKY, 0. “Immobilized Enzymes”, CRC Press, Cleveland, 1973.Google Scholar
  2. 2.
    KEYES, M.H. “Electrodeposition of Enzymes”, U.S. Patent 3,839,175, Oct. 1, 1974.Google Scholar
  3. 3.
    KEYES, M.H. SEMERSKY, F.E. “Polysaccharide Cyclic Carbamate Containing Compounds” U.S. Patent 3,833,555, Sept. 3, 1974.Google Scholar
  4. 4.
    KEYES, M.H. SEMERSKY, F.E. “Insolubilizing Enzymes with Polystyrene Derivatives” U.S. Patent 3,860,486, Jan. 14, 1975.Google Scholar
  5. 5.
    KEYES, M.H. SEMERSKY, F.E. “Polyacrylamide Derivatives and Method of Insolubilization of Enzymes Therewith”. U.S. Patent 3,839,309, Oct. 1, 1974.Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • M. H. Keyes
    • 1
  • R. Barabino
    • 1
  1. 1.Owens-IllinoisToledoUSA

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