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Enzyme Engineering pp 43–49Cite as

Immobilization of Enzymes by Reductive Alkylation

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Abstract

Reductive alkylation in which Schiff’s bases formed between an aldehyde and an amino group may be reduced with sodium borohydride (NaBH4) or sodium cyanoborohydride is a convenient and gentle method to specifically introduce alkyl groups into proteins at ε-amino groups of lysine (1–4). We have used this reaction to couple enzymes to carbohydrate supports which had been oxidized with sodium metaperiodate (NaI04) including dextran-coated, controlled-pore glass (Dextran-CPG), Glycophase-CPG and Sepharose. These are neutral, hydrophilic supports with a highly porous structure. Glycophase-CPG is porous glass coated with a glyceryl silane (Glass≡Si(CH2)3OCH2CHOHCH2OH).

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References

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Author information

Authors and Affiliations

  1. Department of Biochemistry, The Ohio State University, Columbus, Ohio, USA

    G. P. Royer & F. A. Liberatore

Authors
  1. G. P. Royer
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  2. F. A. Liberatore
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Editor information

Editors and Affiliations

  1. Department of Biochemistry and Biophysics School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    E. Kendall Pye

  2. Research and Development Labs, Corning Glass Works, Corning, New York, USA

    Howard H. Weetall

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© 1978 Springer Science+Business Media New York

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Royer, G.P., Liberatore, F.A. (1978). Immobilization of Enzymes by Reductive Alkylation. In: Pye, E.K., Weetall, H.H. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5163-5_5

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  • DOI: https://doi.org/10.1007/978-1-4757-5163-5_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-5165-9

  • Online ISBN: 978-1-4757-5163-5

  • eBook Packages: Springer Book Archive

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