Immobilization of Enzymes by Reductive Alkylation

  • G. P. Royer
  • F. A. Liberatore


Reductive alkylation in which Schiff’s bases formed between an aldehyde and an amino group may be reduced with sodium borohydride (NaBH4) or sodium cyanoborohydride is a convenient and gentle method to specifically introduce alkyl groups into proteins at ε-amino groups of lysine (1–4). We have used this reaction to couple enzymes to carbohydrate supports which had been oxidized with sodium metaperiodate (NaI04) including dextran-coated, controlled-pore glass (Dextran-CPG), Glycophase-CPG and Sepharose. These are neutral, hydrophilic supports with a highly porous structure. Glycophase-CPG is porous glass coated with a glyceryl silane (Glass≡Si(CH2)3OCH2CHOHCH2OH).


Amino Acid Analysis Porous Glass Sodium Cyanoborohydride Sodium Metaperiodate Trypsin Immobilization 
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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • G. P. Royer
    • 1
  • F. A. Liberatore
    • 1
  1. 1.Department of BiochemistryThe Ohio State UniversityColumbusUSA

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