Studies on the Thermal Enzyme Probe

  • C. L. Cooney
  • J. C. Weaver
  • S. P. Fulton
  • S. R. Tannenbaum

Abstract

The coupling of calorimetry with enzymes as a generalized analytical technique has previously been described by us (1) and others (2–4). Here we describe recent work with immobilized enzymes to produce substrate or cofactor specificity in the thermal enzyme probe (TEP). This device employs an enzyme immobilized to one of two thermal sensors which jointly comprise a differential thermometer. Presently the thermal sensors are thermistors connected into a high-precision Wheatstone bridge circuit. When a substrate(s) or cofactor to be detected participates in the enzyme catalyzed reaction, the local release of heat (the enthalpy change associated with all the reactions including protonization) causes a temperature difference between the two sensors of the differential thermometer.

Keywords

Thermal Sensor Unstirred Layer Cofactor Specificity Enzyme Engineer Common Mode Noise 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Cooney, C.L., Weaver, J.C., M. In “Enzyme Engineering Vol.2” (Ed. E.K. Pye and L.B. Wingard, Jr.), Plenum Press, New York, 1974, p. 411.CrossRefGoogle Scholar
  2. 2.
    Mosbach, K. and Danielson, B. Biochim. Biophys. Acta 364: 140, 1974.CrossRefGoogle Scholar
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    Brown, H.D. “Biochemical Microcalorimetry”, Academic Press, New York, 1969.Google Scholar
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    Mcglothlin, C.D. and Jordan, J. Anal Chem. 47: 786, 1975.CrossRefGoogle Scholar
  5. 5.
    Weaver, J.C., Cooney, C.L., Fulton, S.P., Schuler, P. and Tannenbaum, S.R. Biochim. Biophys. Acta 452: 284, 1976.Google Scholar
  6. 6.
    Fulton, S.P. S.B. Thesis, Department of Physics, Massachusetts Institute of Technology, 1975.Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • C. L. Cooney
    • 1
  • J. C. Weaver
    • 1
    • 2
  • S. P. Fulton
    • 1
  • S. R. Tannenbaum
    • 1
  1. 1.Department of Nutrition and Food ScienceMassachusetts Institute of Technology CambridgeCambridgeUSA
  2. 2.Department of PhysicsMassachusetts Institute of Technology CambridgeCambridgeUSA

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