Studies on the Thermal Enzyme Probe
The coupling of calorimetry with enzymes as a generalized analytical technique has previously been described by us (1) and others (2–4). Here we describe recent work with immobilized enzymes to produce substrate or cofactor specificity in the thermal enzyme probe (TEP). This device employs an enzyme immobilized to one of two thermal sensors which jointly comprise a differential thermometer. Presently the thermal sensors are thermistors connected into a high-precision Wheatstone bridge circuit. When a substrate(s) or cofactor to be detected participates in the enzyme catalyzed reaction, the local release of heat (the enthalpy change associated with all the reactions including protonization) causes a temperature difference between the two sensors of the differential thermometer.
KeywordsThermal Sensor Unstirred Layer Cofactor Specificity Enzyme Engineer Common Mode Noise
Unable to display preview. Download preview PDF.
- 3.Brown, H.D. “Biochemical Microcalorimetry”, Academic Press, New York, 1969.Google Scholar
- 5.Weaver, J.C., Cooney, C.L., Fulton, S.P., Schuler, P. and Tannenbaum, S.R. Biochim. Biophys. Acta 452: 284, 1976.Google Scholar
- 6.Fulton, S.P. S.B. Thesis, Department of Physics, Massachusetts Institute of Technology, 1975.Google Scholar