Abstract
Considerable interest has been fostered in the development and application of immobilized “general ligands” such as the adenine-containing coenzymes. This interest derives from the usefulness of such derivatives in affinity chromatography (1–14) and from their potential applications in enzyme reactors (5,6). The chemical complexity of the adenine nucleotide coenzymes has until recently hampered the development of methods suitable for their immobilization. Nevertheless, despite acute synthetic problems, several chemically characterized polymers have been described (7–9). Most methods have concentrated on derivatization of the adenine moiety of the coenzyme and in particular in substitution at the exocyclic N6-amino group. X-ray data from several dehydrogenases has shown that this N6-amino group protrudes out of a hydrophobic pocket in which the adenosine moiety of the coenzyme is bound (10).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lowe, C.R. & Dean, P.D.G. FEBS Lett. 14: 313, 1971.
Lowe, C.R., Harvey, M.J., Craven, D.B., Hollows, M.E. & Dean, P.D.G. Biochem. J., 133: 507, 1973.
Guilford, H., Larsson, P.-O. & Mosbach, K. Chemica Scripta 2: 165, 1972.
Mosbach, K., Guilford, H., Ohlsson, R. & Scott, M. Biochem. J. Z27: 625, 1972.
Larsson, P.-O. & Mosbach, K. Biotechnol. Bioeng. 13: 393, 1971.
Weibel, M.K., Weetall, H.H. & Bright, H.J. Biochem. Biophys. Res. Commun. 44: 347, 1971.
Lamed, R., Levin, Y. & Wilchek, M. Biochem. Biophys. Acta 304: 231, 1973.
Lindberg, M., Larsson, P.O. & Mosbach, K. Eur. J. Biochem. 40: 187, 1973.
Craven, D.B., Harvey, M.J., Lowe, C.R. & Dean, P.D.G. Eur. J. Biochem. 41: 329, 1974.
Adams, M.J., Buehner, M., Chandrasekhar, K., Ford, G.C., Hackert, M.L., Liljas, A., Rossmann, M.G., Smiley, I.E., Allison, W.S., Everse, J., Kaplan, N.O. & Taylor, S.S. Proc. Nati. Acad. Sci. U.S.A. 70: 1968, 1973.
Lowe, C.R. & Dean, P.D.G. FEBS Lett. 18: 31, 1971.
Hipwell, M.C., Harvey, M.J. & Dean, P.D.G. FEBS Lett. 42: 355, 1974.
Harvey, M.J., Craven, D.B., Lowe, C.R. & Dean, P.D.G. Meth. Enzymol. 34: 242, 1974.
Lowe, C.R. & Mosbach, K. Eur. J. Biochem 49: 511, 1974.
Harvey, M.J., Lowe, C.R., Craven, D.B. & Dean, P.D.G. Eur. J. Biochem. 41: 335, 1974.
Lowe, C.R., Harvey, M.J. & Dean, P.D.G. Eur. J. Biochem. 41: 341, 1974.
Harvey, M.J., Lowe, C.R. & Dean, P.D.G. Eur. J. Biochem. 41: 353, 1974.
Lowe, C.R., Harvey, M.J. & Dean, P.D.G. Eur. J. Biochem. 41: 347, 1974.
Lowe, C.R. & Mosbach, K. Eur. J. Biochem. 52: 99, 1975.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1978 Springer Science+Business Media New York
About this chapter
Cite this chapter
Lowe, C.R. (1978). Factors Concerning the Effectiveness of Affinity Chromatography Using Immobilized Cofactors. In: Pye, E.K., Weetall, H.H. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5163-5_35
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5163-5_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-5165-9
Online ISBN: 978-1-4757-5163-5
eBook Packages: Springer Book Archive