Immobilized Cofactors and Cofactor Fragments in General Ligand Affinity Chromatography and as Active Cofactors

  • K. Mosbach
  • P.-O. Larsson

Abstract

It is known that many enzymes function only in conjunction with a coenzyme (cofactor) which is generally a compound of low molecular weight. Immobilization of these compounds has been the focus of much effort because of their potential as bioaffinity ligands and in the search for immobilized coenzymes retaining “biological activity”, which are readily retrievable for reuse. Since the first use of adenine nucleotide analogs as general ligands in affinity chromatography (1–3) great progress has been made in the few years that have elapsed (4–8,25). In these studies the adenyl moiety has been chosen for coupling since it is a part of many coenzymes such as ATP, NAD(P)+, FAD and HSCoA.

Keywords

Affinity Chromatography Alcohol Dehydrogenase Cholic Acid Dependent Enzyme Iodoacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • K. Mosbach
    • 1
  • P.-O. Larsson
    • 1
  1. 1.Biochemical Division, Chemical CenterUniversity of LundSweden

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