Liquid-Membrane Hollow Fiber Enzyme Reactors

  • W. K. Lo
  • S. Putcha
  • B. U. Kim
  • L. Griffith
  • S. Bissell
  • P. R. Rony

Abstract

The hollow fiber enzyme reactor has continued to attract both experimental and theoretical attention (1–8) since a theoretical study was first published in 1971 (9). A motivating force for the interest in the hollow fiber reactor, other than the fact that it is another object for chemical engineers to model mathematically, is the fact that it is one of the few practical reactors for the “immobilization” of multienzyme-coenzyme mixtures, a subject that is very much at the forefront of enzyme engineering at the present time. Notable is the work of Ford, Chambers and collaborators (1–3), who developed the first workable system for continuous steady-state operation with cofactor-requiring enzymes. These investigators employed a Dow reverse osmosis hollow fiber beaker, which had a rather tight membrane cutoff (200 M.W.), to retain the cofactor. They observed, however, that the permeability of even low molecular weight substrates and products (e.g. ethanol, acetaldehyde, and acetate) was quite small and suggested that a more open membrane, one with a higher molecular weight cutoff, would not only increase the permeability of small molecules, but would expand the capability of the hollow fiber reactor to higher molecular weight substrates and products.

Keywords

Hollow Fiber Liquid Membrane Methyl Acetate Fiber Wall Permeation Coefficient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    FORD, J.R., COHEN, W., CHAMBERS, R.P. 75th National Meeting, Am. Inst. Chem. Engr., Detroit, 1973. Abs. 24a.Google Scholar
  2. 2.
    FORD, J.R. Diss. Abstr. Int. B 33: 3624, 1972.Google Scholar
  3. 3.
    CHAMBERS, R.P., FORD, J.R., ALLENDER, J.H., BARICOS, W.H., COHEN, W. In “Enzyme Engineering” (Ed. E.K. Pye and L.B. Wingard, Jr.) Plenum Press, New York, 1974, p. 195.Google Scholar
  4. 4.
    WATERLAND, L.R., MICHAELS, A., ROBERTSON, C.R. AIChE J. 20: 50, 1974.CrossRefGoogle Scholar
  5. 5.
    LEWIS, W., MIDDLEMAN, S. AIChE J. 20: 1012, 1974.CrossRefGoogle Scholar
  6. 6.
    DAVIS, J.E. BiotechnoZ. Bioeng. 27: 1113, 1974.CrossRefGoogle Scholar
  7. 7.
    GEORGAKIS, G., CHAN, P. C-H., ARIS, R. Biotechnol. Bioeng. Z7: 99, 1974.Google Scholar
  8. 8.
    RONY, P.R. J. Amer. Chem. Soc. 94: 8247, 1972.CrossRefGoogle Scholar
  9. 9.
    RONY, P.R. Biotechnol. Bioeng. Z3: 431, 1971.CrossRefGoogle Scholar
  10. 10.
    GARDNER, C.R., COLTON, C.K., LANGER, R.S., HAMILTON, B.K., ARCHER, M.C. WHITESIDES, G.M. In “Enzyme Engineering” (Ed. E.K. Pye L.B. Wingard, Jr.) Plenum Press, New York, 1974, p. 203.Google Scholar
  11. 11.
    WEIBEL, M.K., FULLER, C.W., STADEL, J.M., BUCKMANN, A.F.E.P., DOYLE, T., BRIGHT, H.J. In “Enzyme Engineering” (Ed. E.K. Pye L.B. Wingard, Jr.) Plenum Press, New York, 1974, p. 203.Google Scholar
  12. 12.
    WEIBEL, M.K. In “Enzyme Engineering” (Ed. E.K. Pye L.B.Wingard, Jr.) Plenum Press, New York, 1974, p. 385.Google Scholar
  13. 13.
    BRIGHT, H.J. In “Immobilized Enzymes for Industrial Reactors” (Ed. R.A. Messing) Academic Press, New York, 1975,p. 137.Google Scholar
  14. 14.
    MOSBACH, K. Batelle-Ohio State Symp. Immobilized Enzymes, Columbus, Ohio, 1973.Google Scholar
  15. 15.
    WYKES, J.R., DUNNILL, P., LILLY, M.D. Biochim. Biophys. Acta 286: 260, 1972.CrossRefGoogle Scholar
  16. 16.
    WYKES, J.R., DUNNILL, P., LILLY, M.D. Biotechnol. Bioeng. Z7: 51, 1975.CrossRefGoogle Scholar
  17. 17.
    LOWRY, O.H., PASSONNEAU, J.V., ROCK, M.K. J. Biol. Chem. 236: 2756, 1961.Google Scholar
  18. 18.
    LO, W. K., M.S. Thesis, Department of Chemical Engineering, Virginia Polytechnic Institute State University. Blacksburg, December, 1975.Google Scholar
  19. 19.
    MAHER, J., RONY, P.R. to be published.Google Scholar
  20. 20.
    AHMAD, S.I., BRANYON, A., RONY, P.R. to be published.Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • W. K. Lo
    • 1
  • S. Putcha
    • 1
  • B. U. Kim
    • 1
  • L. Griffith
    • 1
  • S. Bissell
    • 1
  • P. R. Rony
    • 1
  1. 1.Department of Chemical EngineeringVirginia Polytechnic Institute & State UniversityBlacksburgUSA

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