Biological Properties of Lipopolysaccharides Isolated from Bordetella
Biological activity of lipopolysaccharides (LPSs) separated from Bordetella, i.e., B. pertussis (Bp), B. parapertussis (Bpp) and B. bronchiseptica (Bbs), was determined and compared with that of an Escherichia coli LPS as a control. Two Bp-LPS preparations showed marked biological activities comparable to those of E. coli LPS in terms of lethal toxicity in galactosaminesensitized mice, pyrogenicity in rabbits, mitogenicity in C3H/He spleen cell cultures, macrophage activation and tumor necrosis factor-inducing activity. All the activities except mitogenicity of two Bpp-LPS preparations were lower than or comparable to those of E. coli LPS. Activities stronger than or comparable to those of E. coli LPS were observed in two Bbs-LPS preparations. Among six LPS preparations from Bordetella tested, a Bbs-LPS from L3 strain exhibited the most intensive activities.
KeywordsMacrophage Activation Intensive Activity Bordetella Pertussis Lethal Toxicity Japanese White Rabbit
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- 1.Ayme, G., Caroff, M., Chaby, R., H-Cavaillon, N., Dur, A. D., Moreau, M., Muset, M., Mynard, M. C., Roumiantzeff, M., Schulz, D. and Szabo, L., 1980, Biological activities of fragments derived from Bordetella pertussis endotoxin: isolation of a nontoxic, Shwartzman negative lipid A possessing high adjuvant properties. Infect. Immun. 27: 739.PubMedGoogle Scholar
- 3.Kumazawa, Y., Nakatsuka, M., Takimoto, H., Furuya, T., Nagumo, T., Yamamoto, A., Homma, J. Y., Inada, K., Yoshida, M., Kiso, M. and Hasegawa, A., 1988, Importance of fatty acid substituents of chemically synthesized lipid A-subunit analogs in the expression of immunopharmacological activity. Infect. Immun. 56: 149.PubMedGoogle Scholar