Endotoxin pp 525-535 | Cite as

Immunoadjuvanticity of Endotoxins and Nontoxic Derivatives for Normal and Leukemic Immunocytes

  • H. Friedman
  • T. Klein
  • S. Specter
  • C. Newton
  • A. Nowotny
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


Endotoxins from many Gram negative bacteria are known to be immunostimulators. For example, administration of crude or even purified lipopolysaccharide (LPS) endotoxin preparations into experimental animals challenged with various soluble and/or particulate antigens may result in altered immune responses, including enhanced responses, especially when the endotoxins are given shortly before or simultaneously with antigen (4, 10, 14, 15, 17, 19, 24, 25, 26). Studies in these laboratories have shown that a polysaccharide (PS)-rich derivative from endotoxins lacking the lipid A moiety and free of toxicity is a potent immunostimulant for normal mice (7, 8, 11). For example, the PS obtained from Serratia marcescens is equivalent to the intact endotoxin in enhancing the antibody response to a T cell dependent antigen such as sheep erythrocytes (SRBC) in vitro. In vivo the PS was less effective than LPS in enhancing the immune response to SRBC but still considerable adjuvant effect could be demonstrated (1, 2). Injection of SRBC into normal mice or addition of this antigen to cultures of splenocytes from non-immunized mice results in an antibody response readily detected by the hemolytic plaque assay in vitro. Furthermore, treatment of the animals or cell cultures with either LPS or its PS derivative results in enhanced antibody formation.


Antibody Response Normal Mouse Spleen Cell Serratia Marcescens Sheep Erythrocyte 
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  1. 1.
    Behling, U. H., and Nowotny, A., 1977, Immune adjuvancy of lipopoly-saccharide and a nontoxic hydrolytic product demonstrating oscillating effect with time. J. Immunol. 118: 1905–1907.Google Scholar
  2. 2.
    Behling, U. H., Pham, P. H., Madani, F., and Nowotny. A., 1979, Components of lipopolysaccharide-which induce colony stimulation, adjuvancy and radioprotection, in: “Microbiology,” D. Schlessinger, ed. p. 103–107.Google Scholar
  3. 3.
    Bendinelli, M., Matteuci, P., and Friedman, H. 1986. Retrovirus-induced acquired immunodeficiencies. Ad. Canc. Res. 46: 78.Google Scholar
  4. 4.
    Berry, L. J., 1978, The mediation of endotoxemia effects, in: “Toxins,” P. Rosenberg, ed., Pergamon Press. New York.Google Scholar
  5. 5.
    Boivin, A., Mesrobeanu, J., and Mesrobeanu, L., 1933, Extration d’un complexe toxique et antigenique a partier du bacille d’aertrycke. C.R. Soc. Biol. 114: 307.Google Scholar
  6. 6.
    Butler, R. C., Friedman, H., and Nowotny, A., 1980, Restoration of depressed antibody responses of leukemic splenocytes treated with LPSinduced factors. Adv. Exp. Med. Biol. 121: 315.Google Scholar
  7. 7.
    Butler, R. C., Friedman, H., Specter, S. and Eisenstein. T. K., 1981, Induction of immunoenhancing factors for murine splenocyte cultures by Salmonella typhosa ribosome and RNA extracts. Infect. Immun.Google Scholar
  8. 8.
    Frank, S. J., Specter, S., Nowotny, A., and Friedman, H., 1977, Immunocyte stimulation in vitro by nontoxic bacterial lipopolysaccharide derivatives. J. Immunol. 119: 855.Google Scholar
  9. 9.
    Friedman, H. and Specter, S., 1980, Virus induced immunomodulation, in: “Immunopharmacology,” L. Chedid and J. Hadden, eds., Pergamon Press, New York.Google Scholar
  10. 10.
    Friedman, H., Klein, T. W., and Szentivanyi, A., eds., 1981, “Immunomodulation by Bacteria and their Products,” Plenum Press, New York.Google Scholar
  11. 11.
    Friedman, H., Specter, S., and Butler, R. C., 1983, Stimulation of immunomodulatory factors by bacterial endotoxin and nontoxic polysaccharide, in: “Beneficial Effects of Endotoxins,” A. Nowotny, ed., Plenum Press, New York.Google Scholar
  12. 12.
    Friedman, H., Specter, S., and Bendinelli, M., 1984, Viruses and the immune response, in: “Bacterial and Viral Inhibition and Modulation of Host Defenses,” Academic Press, London.Google Scholar
  13. 13.
    Friedman, H., Szentivanyi, A., Specter, S. and Bendinelli, M., 1986, Virus interactions with the immune system, in: “Viruses, Immunity and Immunodeficiencies,” Plenum Press, pp. 25.Google Scholar
  14. 14.
    Homma, J. Y., Kanegasaki, S., Luderitz, 0., Shiba, T., and Westphal, 0., eds., 1984, Bacterial Endotoxins, pp. 1–420, Verlag Chemie, Basel, Switzerland.Google Scholar
  15. 15.
    Klein, T. W., Specter, S., Friedman, H., and Szentivanyi, A., eds., 1983, “Biological Response Modifiers in Human Oncology and Immunology,” Plenum Press, New York.Google Scholar
  16. 16.
    Lachman, L. L., 1983, Interleukin 1 release from LPS-stimulated mononuclear phagocytes, in: “Beneficial Effects of Endotoxins,” A. Nowotny, ed., Plenum Press.Google Scholar
  17. 17.
    Nauts, H. C., 1946, The beneficial effects of bacterial injections on host resistance to cancer. N.Y. Cancer Res. Inst. Monograph 8.Google Scholar
  18. 18.
    Nowotny, A., 1963, Endotoxoid preparations. Nature 197: 721.CrossRefGoogle Scholar
  19. 19.
    Nowotny, A., Golub, S., and Key, B., 1971, Fate and effect of endotoxin derivatives in tumor bearing mice. Proc. Soc. Exp. Biol. Med. 132: 26.Google Scholar
  20. 20.
    Nowotny, A., Behling, U. H., and Chang, H. L., 1975, Relation of structure to function in bacterial endotoxins. VIII. Biological activities in a polysaccharide-rich fraction. J. Immunol. 115: 199–203.Google Scholar
  21. 21.
    Nowotny, A., 1979, in: “Basic Exercises in Immunochemistry,” 2nd edition, Springer Verlag Heidelberg, New York.CrossRefGoogle Scholar
  22. 22.
    Prager, M. D., Ludden, C. M., Landy, W. J., Allison, J. P., and Kelto, G. B., 1975, Endotoxin stimulated immune response to modified lymphoma cells. J. Natl. Canc. Inst. 75: 773.Google Scholar
  23. 23.
    Specter, S. and Friedman, H., 1978, Viruses and immune responses. Pharm. Ther. A 2: 595.Google Scholar
  24. 24.
    Szentivanyi, A., Middleton, E., Williams, J. F., and Friedman, H., 1983, Effect of microbial agents on the immune network and associated pharmacologic reactivities, in: “Allergy: Principles and Practice,” Second Edition, E. Middleton, C. E. Reed, and E. F. Ellis, eds., The C. V. Mosby Company, St. Louis, MO.Google Scholar
  25. 25.
    Szentivanyi, A. and Friedman, H., eds., 1986, “Viruses, Immunity and Immunodeficiency,” Plenum Press, New York.Google Scholar
  26. 26.
    Yang, C. and Nowotny, A., 1974, Effect of endotoxin on tumor resistance in mice. Infect. Immun. 9: 95.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • H. Friedman
    • 1
    • 2
  • T. Klein
    • 1
    • 2
  • S. Specter
    • 1
    • 2
  • C. Newton
    • 1
    • 2
  • A. Nowotny
    • 1
    • 2
  1. 1.University of South Florida College of MedicineTampaUSA
  2. 2.University of PennsylvaniaPhiladelphiaUSA

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