Endotoxin pp 407-412 | Cite as

Lipopolysaccharide-Containing Cytoplasmic Membranes as Immunostimulators of the Peritoneal Macrophages

  • E. Ivanova
  • J. Gumpert
  • A. Popov
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


During the last years we established that the stable L-forms of S. aureus, L. monocytogenes and E. coli were able to survive and multiply inside peritoneal macrophages of experimental animals (11). Our recent studies showed that the cytoplasmic membranes of L-form cells of E. coli WF+ contained lipopolysaccharide (LPS) (8) and induce more pronounced immunostimulating effect on antigen-binding spleen T-lymphocytes compared to cells of the parent bacterium (6) and also compared to an equivalent amount of E. coli lipopolysaccharide (7).


Bactericidal Activity Peritoneal Macrophage Cytoplasmic Membrane Electron Microscopic Observation Mycobacterium Avium 
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  1. 1.
    Bradley, S. G., 1979, Cellular and molecular mechanisms of action of bacterial endotoxins. Ann. Rev. Microbiol. 33: 67–94.Google Scholar
  2. 2.
    Baykousheva, S., Ivanova, E., Gumpert, J. and Toshkov, As., 1980, On the lipid composition of a stable L-form of Escherichia coli W 1655 F+. Acta Microbiol. Bulg. 6: 11–16.Google Scholar
  3. 3.
    Frehell, C., de Chastellier, C., Lang, T. and Rastogi, N., 1986, Evidence for inhibition of fusion of lysosomal and prelysosomal compartments in macrophages infected with pathogenic Mycobacterium avium. Infect. Immun., No. 1, 52: 252–262.Google Scholar
  4. 4.
    Gumpert, J. Schuhmann, E. and Taubeneck, U., 1971, Ultrastruktur der stabilen L-Formen von Escherichia coli B and W 1655 F+. Z. Allg. Mikrobiologie 11: 19–33.Google Scholar
  5. 5.
    Ivanova, E. and Gumpert, J., 1986, Use of cytoplasmic membranes of bacterial L-forms as immunostimulators. Invention Reg. No. 75653, 8. 07.Google Scholar
  6. 6.
    Ivanova, E., Slavcheva, E., Gumpert, J. and Georgiev, D. 1988, Cytoplasmic membranes as activators of T-cell population, VIth Congress of Immunol., Toronto, Canada, 6-11 July 1986. Abstracts, p. 332; Epidemio, Microbiol. and Inf. Dis. (in press).Google Scholar
  7. 7.
    Ivanova, E., Slavcheva, E., Gumpert, J., Georgiev, D. and Stojanova, P., 1988, Cytoplasmic membranes and lipopolysaccharide of Escherichia coli as immunostimulators. Acta Microbiol. Bulg. (in press).Google Scholar
  8. 8.
    Michailova, L., Ivanova, E., Gumpert, J., Toshkov, As. and Jordanova, M., 1984, Immunoelectron microscopic determination of 0-antigen localization in stable L-forms of Escherichia coli. C. R. Acad. Bulg. Sci. No. 1, 37: 77–80.Google Scholar
  9. 9.
    Nathan, C. F., Murray, H. W. and Cohn, Z. A., 1980, The macrophage as an effector cell. N. Engl. J. Med. 303: 622.Google Scholar
  10. 10.
    Osborn, M. J., Gander, J. E., Parisie, E. and Carson, J., 1972, Mechanism of assembly of the outer membrane. II. Site of synthesis of lipopolysaccharide. J. Biol. Chem. 247: 3973–3986.Google Scholar
  11. 11.
    Schmitt-Slomska, J., Michailova, L., Ivanova, E. and Toshkov, As., 1986, Adhesion and phagocytosis of Staphylococcus aureus L-forms. J. Basic Microbiol. No. 7, 26: 429–440.CrossRefGoogle Scholar
  12. 12.
    Toshkov, As. and Dimov, V., Immunostimulation and immunostimulators, actual problems in science. Bulg. Acad. Sci., XII, No. 8.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • E. Ivanova
    • 1
  • J. Gumpert
    • 2
  • A. Popov
    • 3
  1. 1.Institute of MicrobiologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Central Institute of Microbiology and Experimental TherapyJenaGermany
  3. 3.Scientific Medico-Biological InstituteMedical AcademySofiaBulgaria

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