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Lipopolysaccharide-Induced Priming of the Murine Macrophage-Like Cell Line J774A.1 for Enhanced Production of Reactive Oxygen Intermediates is Blocked by Antiserum to Murine Interferon β

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Endotoxin

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 256))

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Abstract

Previously, we have shown that lipopolysaccharide (LPS) can prime murine macrophage-like J774A.1 cells for an enhanced production of reactive oxygen intermediates (ROI) in a dose- and time-dependent manner. The goal of this study was to determine if cytokines produced and secreted by LPS-treated J774A.1 cells were involved in priming for enhanced production of ROI. This was accomplished by neutralizing cytokine activity using specific anti-cytokine antiserum added to the LPS-treated J774A.1 cells.

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References

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Author information

Authors and Affiliations

  1. Department of Medical Microbiology, University of Wisconsin Medical School, Madison, Wisconsin, USA

    T. P. Birkland & R. A. Proctor

Authors
  1. T. P. Birkland
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  2. R. A. Proctor
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Editor information

Editors and Affiliations

  1. The University of South Florida, Tampa, Florida, USA

    Herman Friedman  & T. W. Klein  & 

  2. Jichi Medical School, Tochigi-Ken, Japan

    Masayasu Nakano

  3. University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Alois Nowotny

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© 1990 Springer Science+Business Media New York

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Birkland, T.P., Proctor, R.A. (1990). Lipopolysaccharide-Induced Priming of the Murine Macrophage-Like Cell Line J774A.1 for Enhanced Production of Reactive Oxygen Intermediates is Blocked by Antiserum to Murine Interferon β. In: Friedman, H., Klein, T.W., Nakano, M., Nowotny, A. (eds) Endotoxin. Advances in Experimental Medicine and Biology, vol 256. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5140-6_36

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  • DOI: https://doi.org/10.1007/978-1-4757-5140-6_36

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-5142-0

  • Online ISBN: 978-1-4757-5140-6

  • eBook Packages: Springer Book Archive

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