Endotoxin pp 403-406 | Cite as

Lipopolysaccharide-Induced Priming of the Murine Macrophage-Like Cell Line J774A.1 for Enhanced Production of Reactive Oxygen Intermediates is Blocked by Antiserum to Murine Interferon β

  • T. P. Birkland
  • R. A. Proctor
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


Previously, we have shown that lipopolysaccharide (LPS) can prime murine macrophage-like J774A.1 cells for an enhanced production of reactive oxygen intermediates (ROI) in a dose- and time-dependent manner. The goal of this study was to determine if cytokines produced and secreted by LPS-treated J774A.1 cells were involved in priming for enhanced production of ROI. This was accomplished by neutralizing cytokine activity using specific anti-cytokine antiserum added to the LPS-treated J774A.1 cells.


Tumor Necrosis Factor Reactive Oxygen Intermediate Recombinant Human Tumor Necrosis Recombinant Tumor Necrosis Factor Recombinant Human Tumor Necrosis Factor 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • T. P. Birkland
    • 1
  • R. A. Proctor
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of Wisconsin Medical SchoolMadisonUSA

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