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Endotoxin pp 403-406 | Cite as

Lipopolysaccharide-Induced Priming of the Murine Macrophage-Like Cell Line J774A.1 for Enhanced Production of Reactive Oxygen Intermediates is Blocked by Antiserum to Murine Interferon β

  • T. P. Birkland
  • R. A. Proctor
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)

Abstract

Previously, we have shown that lipopolysaccharide (LPS) can prime murine macrophage-like J774A.1 cells for an enhanced production of reactive oxygen intermediates (ROI) in a dose- and time-dependent manner. The goal of this study was to determine if cytokines produced and secreted by LPS-treated J774A.1 cells were involved in priming for enhanced production of ROI. This was accomplished by neutralizing cytokine activity using specific anti-cytokine antiserum added to the LPS-treated J774A.1 cells.

Keywords

Tumor Necrosis Factor Reactive Oxygen Intermediate Recombinant Human Tumor Necrosis Recombinant Tumor Necrosis Factor Recombinant Human Tumor Necrosis Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Borashi, D., Ghezzi, P., Salmona, M., and Tagliabue, A., 1982, IFNβ-induced reduction of superoxide anion generation by macrophages. Immunol. 45: 621.Google Scholar
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • T. P. Birkland
    • 1
  • R. A. Proctor
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of Wisconsin Medical SchoolMadisonUSA

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