Endotoxin pp 361-368 | Cite as

The Role of 13-Hydroxylinoleic Acid in the Activation of Macrophages by Lipopolysaccharide

  • U. F. Schade
  • I. Burmeister
  • R. Engel
  • H. Lode
  • I. Kozka
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


Bacterial lipopolysaccharides (LPS, endotoxin) are known to activate various functions and morphological properties of mononuclear phagocytes both in vivo and in vitro (11, 15). Recently, it has been reported that phagocytosis in bone marrow derived macrophage cultures was stimulated with LPS, indicating a direct interaction between endotoxin and the cells (4). The mechanism of the activation of monocytes is unknown. However, some LPS induced phenomena require the presence of arachidonic acid metabolites, e.g., the LPS-induced production of collagenase (1) and the release of interleukin 1 (5). In order to determine the possible role of cyclooxygenases and lipoxygenases in the activation of macrophages by LPS, the effects of several inhibitors of these enzymes on the binding and uptake of zymosan particles by macrophages primed with LPS was investigated and a lipoxygenase product, 13-hydroxyoctadecadienoic acid (13-HODD) was isolated from activated cells. Addition of exogenous 13-HODD led to increased association/uptake of zymosan with the cells. Therefore, our results suggest that 13-HODD is possibly involved in the activation process of macrophages by LPS.


Mouse Peritoneal Macrophage Association Index Lipoxygenase Product Soybean Lipoxygenase Eicosatetraenoic Acid 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • U. F. Schade
    • 1
  • I. Burmeister
    • 1
  • R. Engel
    • 1
  • H. Lode
    • 1
  • I. Kozka
    • 1
  1. 1.Institut fur Experimentelle Medizin und BiologieForschungsinstitut BorstelBorstelGermany

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