Endotoxin pp 347-359 | Cite as

Possible Refractory Site on LPS-Induced Interleukin 1 Production in C3H/HeJ Peritoneal Macrophages

  • M. Nakano
  • Y. Terada
  • H. Matsumura
  • H. Shinomiya
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


The C3H/HeJ strain of mice is known to be unresponsive to LPS (20), and this peculiar characteristic of these mice has greatly contributed to the analysis of the complex processes involved in the cell activation by LPS as a negative control. The unresponsiveness of these mice is believed to be attributed to a mutation of a single gene locus on chromosome 4 that has been designated as the LPS-gene with normal (n) and defective (d), alleles, respectively (26). Because C3H/HeJ mice carry the LPS-defective gene (Lpsd), their macrophages cannot secrete interleukin 1 (IL-1) in response to LPS (21). Determining the location of where the blocking sites are phenotypically expressed by the defective gene may provide us with a useful approach for elucidating the triggering by LPS, because it must be one of the important sites of this pathway. In a previous paper (22), we demonstrated that the C3H/HeJ macrophages are unresponsive to the calcium ionophore A23187 as well as LPS, ang we suggested that the blocking sites expressed phenotypically by the Lps are shared by LPS- and A23187-stimulated processes. In the present study, we intend to elucidate the difference of intracellular signal transmission on the LPS-induced IL-1 production in macrophages between LPS-responsive C3H/He mice and LPS-unresponsive C3H/HeJ mice.


Calcium Ionophore Peritoneal Exudate Cell Calcium Ionophore A23187 Human Peripheral Blood Monocyte Blocking Site 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • M. Nakano
    • 1
  • Y. Terada
    • 1
  • H. Matsumura
    • 1
  • H. Shinomiya
    • 1
  1. 1.Department of MicrobiologyJichi Medical SchoolTochigiJapan

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