Endotoxin pp 287-299 | Cite as

Investigation of Endotoxin Binding Cationic Proteins from Granulocytes; Agglutination of Erythrocytes Sensitized with Re-LPS

  • M. Hirata
  • M. Yoshida
  • K. Inada
  • T. Kirikae
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


Endotoxin has been found to cause cytotoxic damages of mouse bone marrow cells, especially granulocytes and macrophages, migration of marrow cells into circulation (3, 18–20), and increase in the tissue factor (tissue thromboplastin) activity in these cells (4–6). Tissue factor activity is found on the membrane surface of intact cells (i.e., cell suspension), and homogenization or sonic disruption of the cells weakened the tissue factor activity. This phenomenon is due to anticoagulant activity of cationic proteins (CAP) released from these cells (5, 7, 15). CAP from mouse bone marrow or rabbit granulocytes are the endotoxin (LPS)-binding proteins since (a) the mixture of LPS and CAP solution increased turbidity, and (b) preincubation of LPS with CAP inhibited some LPS activities (activation of blood coagulation factor XII, and lethality to mouse and tissue factor generation in mouse spleen cells) (5). Recently CAP was found to cause agglutination of erythrocytes sensitized with LPS, especially Re-LPS and lipid A.


Anticoagulant Activity Mouse Bone Marrow Cationic Protein Sheep Erythrocyte Fast Protein Liquid Chromatography 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • M. Hirata
    • 1
  • M. Yoshida
    • 1
  • K. Inada
    • 1
  • T. Kirikae
    • 1
  1. 1.Department of Bacteriology, School of MedicineIwate Medical UniversityMirioka, 020 IwateJapan

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