Abstract
Endotoxin has been found to cause cytotoxic damages of mouse bone marrow cells, especially granulocytes and macrophages, migration of marrow cells into circulation (3, 18–20), and increase in the tissue factor (tissue thromboplastin) activity in these cells (4–6). Tissue factor activity is found on the membrane surface of intact cells (i.e., cell suspension), and homogenization or sonic disruption of the cells weakened the tissue factor activity. This phenomenon is due to anticoagulant activity of cationic proteins (CAP) released from these cells (5, 7, 15). CAP from mouse bone marrow or rabbit granulocytes are the endotoxin (LPS)-binding proteins since (a) the mixture of LPS and CAP solution increased turbidity, and (b) preincubation of LPS with CAP inhibited some LPS activities (activation of blood coagulation factor XII, and lethality to mouse and tissue factor generation in mouse spleen cells) (5). Recently CAP was found to cause agglutination of erythrocytes sensitized with LPS, especially Re-LPS and lipid A.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Carr, C. and Morrison, D. C., 1984, Lipopolysaccharide interaction with rabbit erythrocyte membranes. Infect. Immun. 43: 600–606.
Ganz, T., Selsted, M. E., Szklarek, D., Earwig, S. S. L., Daher, K., Baiton, D. F. and Lehrer, R. I., 1985, Defensins: Natural peptide antibiotics of human neutrophils. J. Clin. Invest. 76: 1427–1435.
Hirata, M., 1975, Inhibitory effects of antihistamines and antiserotonins on the bone marrow reactions produced by Escherichia coli endotoxin in mice. J. Inf. Dis. 132: 611–616.
Hirata, M., Inada, K., Tsunoda, N. and Yoshida, M., 1980, Relationship between cells in bone marrow and intravascular coagulation in endotoxicosis, in: “Bacterial Endotoxins and Host Response”, M. K. Argarwal, ed., Elsevier/North-Holland Biomedical Press, p. 255–272.
Hirata, M., Tsunoda, N., Suzuki, Y., Inada, K. and Yoshida, M., 1982, Endotoxemia and blood coagulation. 2. Anticoagulant activities of mouse bone marrow and spleen cells. Blood and Vessel. 13: 237–240.
Hirata, M., Yoshida, M., Tsunoda, N. and Inada, K., 1984, Endotoxemia and blood coagulation; Procoagulant activity of mouse bone marrow cells, in: “Bacterial Endotoxin: Chemical, Biological and Clinical Aspects”, J. Y. Homma, S. Kanegasaki, O. Lüderitz, T. Shiba and 0. Westphal, eds., Verlag Chemie p. 351–362.
Hirata, M., Tsunoda, N. and Yoshida, M., 1987, Anticoagulant, cationic protein (CAP) isolated from granulocytes: Endotoxin-binding property of CAP, Blood and Vessel. 18: 592–594.
Kirikae, T., Inada, K., Hirata, M., Yoshida, M., Galanos, C. and Lúderitz, 0., 1986, Hemagglutination induced by lipopolysaccharides and lipid A. Microbiol. Immunol. 30: 269–274.
Lehrer, R. I., Selsted, M. E., Szklarek, D. and Fleischmann, J., 1983, Antibacterial activity of microbicidal cationic proteins 1 and 2, Natural peptide antibiotics of rabbit lung macrophages. Infect. Immun. 42: 10–14.
Morrison, D. C. and Jacob, D. M., 1976, Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 13: 813–818.
Morita, T., Ohtsubo, S., Nakamura, T., Tanaka, S., Iwanaga, S., Ohashi, K. and Niwa, M., 1985, Isolation and biological activities of limulus anticoagulant (anti-LPS factor) which interact with lipopolysaccharide (LPS). J. Biochem. 97: 1611–1620.
Ohashi, K., Niwa, M., Nakamura, T., Morita, T. and Iwanaga, S., 1984, Anti-LPS factor in the horseshoe crab, Tachypleus tridentatus: Its hemolytic activity on the red blood cell sensitized with lipopolysaccharide. FEBS Letters. 176: 207–210.
Oderberg, H. and Olsson, I., 1975, Antibacterial activity of cationic proteins from human granulocytes. J. Clin. Invest. 56: 1118–1124.
Oroszlan, S. I., Mora, R. T. and Shear, M. J., 1963, Reversible inactivation of an endotoxin by intracellular protein. Biochem. Pharmacol. 12: 1131–1146.
Saba, H. I., Roberts, H. R. and Herion, J. C., 1967, The anticoagulant activity of lysosomal cationic proteins from polymorphonuclear leukocytes. J. Clin. Invest. 46: 580–589.
Selsted, M. E., Szklarek, D. and Lehrer, R. I., 1984, Purification and antibacterial activity of antimicrobial peptides of rabbit granulocytes. Infect. Immun. 45: 150–154.
Shafer, W. M., Martin, L. E. and Spitznagel, J. K., 1984, Cationic antimicrobial proteins isolated from human neutrophil granulocytes in the presence of diisopropyl fluorophosphate. Infect. Immun. 45: 29–35.
Yoshida, M., Hirata, M., Hatano, Y. and Inada, K., 1968, Hemorrhagic necrosis in mouse bone marrow induced by a single injection of endotoxin, and its application to the bioassay of endotoxin and to the study on biological activity of K-antigen. Japan J. Exp. Med. 38: 335–346.
Yoshida, M., Hirata, M. and Agarwal, M. K., 1976, Study on in vivo cytotoxicity caused by endotoxin injection, in: “Animal, Plant, and Microbial Toxins”, A. Osaka, K. Hayashi and Y. Sawai, eds., Plenum Publishing Co., New York, p. 509–520.
Yoshida, M., Hirata, M. and Inada, K., 1973, Hemorrhage and necrosis in mouse bone marrow induced by endotoxin: A method assaying quantitative changes of the cellularities. Japan J. Exp. Med. 43: 393–402.
Yoshida, M., Kudoh, K., Hirata, M., Inada, K. and Ogasawara, M., 1984, Neutralizing effects of anti-Salmonella Re-antibody on endotoxic bone marrow reactions and induction of procoagulant activity, in: “Immunopharmacology of Endotoxicosis”, M. K. Agarwal and M. Yoshida, eds., Walter de Gruyter, Berlin, p. 77–92.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media New York
About this chapter
Cite this chapter
Hirata, M., Yoshida, M., Inada, K., Kirikae, T. (1990). Investigation of Endotoxin Binding Cationic Proteins from Granulocytes; Agglutination of Erythrocytes Sensitized with Re-LPS. In: Friedman, H., Klein, T.W., Nakano, M., Nowotny, A. (eds) Endotoxin. Advances in Experimental Medicine and Biology, vol 256. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5140-6_25
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5140-6_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-5142-0
Online ISBN: 978-1-4757-5140-6
eBook Packages: Springer Book Archive