Primary Structures and Functions of Anti-Lipopolysaccharide Factor and Tachyplesin Peptide Found in Horseshoe Crab Hemocytes
The component of lipopolysaccharide (LPS) located in the cell surface of Gram-negative bacteria, has various biological activities, such as pyrogenicity, adjuvant activity, activation of macrophage and B lymphocyte antitumor activity, and so on (19). It is also known that LPS induces the activation and degranulation of horseshoe crab hemocytes and results in the hemolymph coagulation (2, 7). This cellular event is thought to be one of the self-defense mechanisms of horseshoe crab (12, 18, 23). The recent studies on the limulus coagulation system indicate that it consists of the sequential activations of at least three serine protease zymogens and subsequent conversion of coagulogen, an invertebrate fibrinogen-like substance, to coagulin gel (9, 10, 14–16, 21).
KeywordsHorseshoe Crab Cationic Peptide Double Diffusion Hemocyte Lysate Tachypleus Tridentatus
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