Endotoxin pp 225-229 | Cite as

A New Perchloric Acid Treatment of Human Plasma for Detection of Endotoxin by an Endotoxin-Specific Chromogenic Test

  • K. Inada
  • M. Yoshida
  • T. Takahashi
  • S. Tamura
  • S. Tanaka
  • S. Endo
  • T. Yoshida
  • H. Suda
  • T. Komuro
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)

Abstract

Recently, an endotoxin-specific colorimetric assay method, using synthetic chromogenic substrate, was developed using factor G-depleted lysate (Endospecy, Seikagaku Kogyo)(3). Since many interfering factors and inhibitors of Limulus amebocyte lysate test are present in human plasma, it became necessary to remove these prior to the detection of endotoxin. Perchloric acid (PCA) treatment of human plasma has been used to inactivate or remove the interfering factors (2). In this method, PCA is added to test plasma and the mixture is incubated. The precipitate is discarded after centrifugation. The supernantant is employed for detection of endotoxin using Endospecy. We have found, however, that a large amount of endospecy activity was recovered from the precipitate, and we developed an improved method of PCA treatment by which endotoxin content in both the precipitate and supernatant could be measured.

Keywords

Chromogenic Substrate Endotoxin Level Patient Plasma Lethal Toxicity Endotoxin Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Obayashi, T., 1984, Addition of perchloric acid to blood samples for colorimetric Limulus test using chromogenic substrate. J. Lab. Clin. Med. 104: 321–330.PubMedGoogle Scholar
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    Obayashi, T., Tamura, H., Tanaka, S., Ohki, M., Takahashi, S., Arai, M., Masuda, M. and Kawai, T., 1985, A new chromogenic endotoxin-specific assay using recombined Limulus coagulation enzymes and its clinical applications. Clin. Chim. Acta 149: 55–65.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • K. Inada
    • 1
  • M. Yoshida
    • 1
  • T. Takahashi
    • 1
  • S. Tamura
    • 2
  • S. Tanaka
    • 2
  • S. Endo
    • 3
  • T. Yoshida
    • 4
  • H. Suda
    • 5
  • T. Komuro
    • 6
  1. 1.Department of Bacteriology, School of MedicineIwate Medical UniversityMorioka 020Japan
  2. 2.Tokyo Research InstituteSeikagaku Kogyo Co. Ltd.Tokyo 189Japan
  3. 3.Critical Care and Emergency Center, School of MedicineIwate Medical UniversityMorioka 020Japan
  4. 4.1st Department of Internal Medicine, School of MedicineIwate Medical UniversityMorioka 020Japan
  5. 5.Department of Pediatrics, School of MedicineIwate Medical UniversityMorioka 020Japan
  6. 6.National Institute of Hygiene (Osaka)520 OsakaJapan

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