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Chemistry and Biology of a Novel Lipid Contaminant of Some Endotoxin Preparations with Selective Cytotoxicity to Transformed Cells

  • Chapter
Endotoxin

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 256))

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Abstract

The heterogeneity of various endotoxin (ET) preparations, should they be extracted by the trichloroacetic acid method of Boivin and co-workers, or by the phenol-water procedure of Westphal and Luderitz, has been well documented (26, 27). Several papers from our laboratory elaborated on the experimental details and emphasized the significance of this observation from both chemical and biological viewpoints (23, 24, 25, 29).

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References

  1. Blum, S. T., Shohet, S. B., Nathan, D. G., and Gardner, F. H., 1969, The effect of amphotericin B on erythrocyte membrane cation permeability: its relation to in vivo erythrocyte survival. J. Lab. Clin. Med. 73: 980.

    Google Scholar 

  2. Butler, R. C., and Nowotny, A., 1979. Combined immunostimulation in the prevention of tumor take in mice using endotoxins, their derivatives and other immune adjuvants. Cancer Immunol. Immunotherapy. 6: 255.

    Google Scholar 

  3. Buttke, T. M., and Ingram, L. 0., 1975, Comparison of lipopolysaccharides from Agmenellus quadriplicatum to Bscherichia coli and Salmonella typhimurium by using thin-layer chromatography. J. Bacteriol. 124: 1566.

    CAS  Google Scholar 

  4. Chang, C., Simmons, D. T., Martin, M. A., and Mora, P. T., 1979, Identification and partial characterization of new antigens from simian virus 40-transformed mouse cells. J. Virol. 31: 463.

    Google Scholar 

  5. Chang, C. M., and Nowotny, A., 1975, Relation of structure to function in bacterial 0-antigens VII. Endotoxicity of ‘Lipid A’. Immunochem. 12: 19.

    CAS  Google Scholar 

  6. Chen, C. H., Chang, C. M., Nowotny, A. M., and Nowotny, A., 1975, Rapid biological and chemical analysis of bacterial endotoxins separated by preparative thin-layer chromatography. Anal. Biochem. 63: 183.

    Google Scholar 

  7. Chen, C. H., Johnson, A. G., Kasai, N., Key, B. A., Levin, J., and Nowotny, A., 1973, Heterogeneity and biological activity of endotoxin glycolipid from Salmonella minnesota R595. J. Infect. Dis. 128: s43.

    Google Scholar 

  8. Chester, I. R., and Meadow, P. M., 1975, Heterogeneity of lipopolysaccharide from Psuedomonas aeruginosa Eur. J. Biochem. 58: 621.

    Google Scholar 

  9. Coley, W. B., 1985, The treatment of inoperable malignant tumors with the toxins of erysipelas and Bacillus prodigiosus Med. Rec. 47: 65.

    Google Scholar 

  10. Dye, E. S., and North, R. J., 1980, Macrophage accumulation in murine ascites tumors. I. Cytoxan-induced dominance of macrophages over tumor cells and the antitumor effect of endotoxin. J. Immunol. 125: 1650.

    Google Scholar 

  11. Freid, M., and Prives, C., 1986, The biology of simian virus 40 and polyomer virus, j “Cancer cells IV. DNA tumor viruses”, M. Botchan, E. Grubzicker, P. A. Sharp, eds., Cold Spring Harbor Laboratory, NY.

    Google Scholar 

  12. Friedman, H., Blanchard, D. K., Newton, C., Klein, T., Stewart II, W., Keler, T., and Nowotny, A., 1987, Distinctive immunomodulatory effects of endotoxin and nontoxic lipopolysaccharide derivatives in lymphoid cell cultures. J. Biol. Response Mod. 6: 664.

    CAS  Google Scholar 

  13. Gmeiner, J., 1975, The isolation of two different lipopolysaccharide fractions from various Proteus mitabilis strains. Eur. J. Biochem. 58: 621.

    Google Scholar 

  14. Grohsman, J., and Nowotny, A., 1972, The immune recognition of TA3 tumors, its facilitation by endotoxin and abrogation by ascites fluid. J. Immunol. 109: 1090.

    Google Scholar 

  15. Jann, B., Reske, K., and Kann, J., 1975, Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Eur. J. Biochem. 60: 239.

    Google Scholar 

  16. Keler, T., Kovats, E., Nguyen, V., Samu, J., Sanavi, F., Somlyo, B., and Nowotny, A., 1987, Cytotoxicity of a novel lipid-like bacterial product. Biochem. Biophys. Res. Comm. 149: 1033.

    Google Scholar 

  17. Kinsky, S. C., 1970, Antibiotic interaction with model membranes. Annu. Rev. Pharmacol. 10: 119.

    Google Scholar 

  18. Krebs, H. A., 1972, The Pasteur effect and the relations between respiration and fermentation, in: “Essays in Biochemistry”, P. N. Campbell and F. Dickens, eds., Academic Press, Vol. 8.

    Google Scholar 

  19. Lampen, J. O., 1966, Interference by antifungal antibiotics (especially nysstatin and filipin) with specific membrane functions, in: “Biochemical studies of antimcrobial drugs”, B. A. Newton, P. E. Reynolds, eds., Cambridge Univ. Press.

    Google Scholar 

  20. McFarland, V. W.. Mora, P. T., Schultz, A., and Pancake, S.. 1975, Cell properties after repeated transplantation of spontaneously and of SV40 virus transformed mouse cell lines. I. Growth in culture. J. Cell Physiol. 85: 101.

    Google Scholar 

  21. Nauts, H. C.. Swift, W. E., and Coley, B. L., 1946, The treatment of malignant tumors by bacterial toxins as developed by the late William B. Coley, M.D., reviewed in the light of modern research. Cancer Res. 1: 731.

    Google Scholar 

  22. Ng, A. K., Butler, C., Chen, C. H., and Nowotny, A., 1976, Relationship of structure to function in bacterial endotoxins IA. Differences in the lipid moiety of endotoxic glycolipids. J. Bacteriol. 126: 511.

    Google Scholar 

  23. Nowotny, A., 1961, Chemical structure of a phosphomucolipid and its occurrence in some strains of Salmonella. J. Am. Chem. Soc. 83: 501.

    Google Scholar 

  24. Nowotny, A., 1963, Relations of structure to function in bacterial 0-antigens II. Fractionation of lipids present in Boivin-type endotoxin of Serratia aarcescens J. Bacteriol. 85: 127.

    Google Scholar 

  25. Nowotny, A., 1966, Heterogeneity of endotoxic bacterial lipopolysaccharides revealed by ion-exchange chromatography. Nature 210: 278.

    CAS  Google Scholar 

  26. Nowotny, A., 1971, Chemical and biological heterogeneity of endotoxins, in: “Microbial Toxins, Vol. 4: Bacterial Endotoxins”, G. Weinbaum, S. Kadis, S. J. Ajl, eds., Academic Press, New York-London.

    Google Scholar 

  27. Nowotny, A., 1984, Heterogeneity of endotoxins, in: “Handbook of Endotoxins, Vol. I”, E. T. Rietschel, ed., Elsevier Science Publishers, Amsterdam.

    Google Scholar 

  28. Nowotny, A., Abdelnoor, A., Behling. U. H., Butler, R. C., Johnson, A. G., and Nowotny, A. M., 1982, Molecular aspects of the adjuvant and other beneficial effects of endotoxins, in: “Immunomodulation by microbial products and related synthetic compounds”, Y. Yamura, S. Kotani. eds., Elsevier Science Publishing Co., Amsterdam.

    Google Scholar 

  29. Nowotny, A., Cundy, K. R., Neale, N. L., Nowotny, A. M., Radvany, R.. Thomas, S. P., and Tripodi. D. J., 1966, Relationship of structure to function in bacterial 0-antigens IV. Fractionation of the components. Ann. N.Y. Acad. Sci. 133: 586.

    CAS  Google Scholar 

  30. Nowotny, A., Keler, T., Pham, P.H., Kovats, E., Aiello, A., Shonekan, 0., Friedman, H., and Johnson, A. G., 1988, Isolation of a nonendotoxic antitumor preparation from Serratia aarcescens. J. Biol. Response Mod. 7: 296.

    CAS  Google Scholar 

  31. Ribi. E., Parker, R., Strain, S. M., Mizuno, Y., Nowotny, A., von Eschen, K. B., Cantrell, J. L., McLaughlin, C. A., Hwand, K. M., and Goren, M. B., 1979, Peptides as requirements for immunotherapy of the guinea-pig line- 10 tumor with endotoxins. Cancer Immunol. Immunotherapy. 7: 43.

    Google Scholar 

  32. Rothman, Y., Johnson, A. G., Friedman, H., Kovats, E., Pham, P. H., Sanavi, F., Nowotny, A. M.. and Nowotny. A., 1985, Biological effects of White-type polysaccharide of Gram-negative bacteria. J. Biol. Response Mod. 4: 169.

    Google Scholar 

  33. Samu, J., Kovats, E., Nguyen, V., Keler, T., and Nowotny, A., 1988, Thin-layer chromatography of endotoxins, their derivatives and contaminants. J. Chromatog. 435: 167.

    Google Scholar 

  34. Schiaffonati, L., and Baserga, R., 1977, Different survival of normal and transformed cells exposed to nutritional conditions nonpermissive for growth. Cancer Res. 37: 541.

    CAS  Google Scholar 

  35. Shear, M. J., 1941, Effect of a concentrate from B. prodigiosus filtrate on subcutaneous primary induced mouse tumors. Cancer Res. 1: 731.

    Google Scholar 

  36. Shear, M. J., and Andervont, H. B., 1936, Chemical treatment of tumors. III Separation of hemorrhage-producing fraction of B. coli filtrate. Proc. Soc. Exp. Biol. Med. 34: 323.

    Google Scholar 

  37. Shear, M. J.. and Turner, E. C., 1943, Chemical treatment of tumors. V. Isolation of hemorrhage-producing fraction from Serratia aarcescens (Bacillus prodigiosus) culture filtrate. J. Natl. Cancer Inst. 4: 81.

    Google Scholar 

  38. Tooze, J., 1980, DNA tumor viruses, in: “Molecular Biology of Tumor Viruses, 2nd ed.”, J. Tooze, ed., Cold Spring Harbor Laboratory, NY.

    Google Scholar 

  39. Winterbourne, D. J.. and Mora, P. T., 1978, Altered metabolism of hepa-ran sulfate in simian virus 40 transformed clonal mouse cells. J. Biol. Chem. 253: 5109.

    Google Scholar 

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Author information

Authors and Affiliations

  1. The Thomas W. Evans Museum and Dental Institute, University of Pennsylvania, Philadelphia, PA, 19104, USA

    T. Keler & A. Nowotny

  2. National Cancer Institute, Bethesda, MD, 20892, USA

    C. A. D. Smith

Authors
  1. T. Keler
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  2. C. A. D. Smith
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  3. A. Nowotny
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Editor information

Editors and Affiliations

  1. The University of South Florida, Tampa, Florida, USA

    Herman Friedman  & T. W. Klein  & 

  2. Jichi Medical School, Tochigi-Ken, Japan

    Masayasu Nakano

  3. University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Alois Nowotny

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© 1990 Springer Science+Business Media New York

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Keler, T., Smith, C.A.D., Nowotny, A. (1990). Chemistry and Biology of a Novel Lipid Contaminant of Some Endotoxin Preparations with Selective Cytotoxicity to Transformed Cells. In: Friedman, H., Klein, T.W., Nakano, M., Nowotny, A. (eds) Endotoxin. Advances in Experimental Medicine and Biology, vol 256. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5140-6_14

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  • DOI: https://doi.org/10.1007/978-1-4757-5140-6_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-5142-0

  • Online ISBN: 978-1-4757-5140-6

  • eBook Packages: Springer Book Archive

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