Endotoxin pp 137-139 | Cite as

Cloning and Expression of rfe Gene

  • M. Ohta
  • N. Kido
  • K. Jann
  • Y. Arakawa
  • T. Komatsu
  • H. Ito
  • N. Kato
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)

Abstract

Bacterial rfe gene is involved in the synthesis of enterobacterial common antigen (ECA) as well as some kind of endotoxins such as lipopolysaccharides (LPS) from Escherichia coli 08, 09, Salmonella typhimurium, and other members of the family Enterobacteriaceae (1, 2, 3, 6). The gene rfe is located close to ilv genes at 84.5 min on the E. coli chromosomal map in the gene order ilv rfe rff uvrD metE (4). In the recent study, it was suggested that the products of the rfe gene(s) function mainly in supporting the coordinated elongation of the 09 mannan chain of E. coli (7). Moreover, Lew and coworkers showed that one of the enzymes coded for by the rfe gene cluster in group Cl salmonellae is dTDPglucose pyrophosphorylase, the function of which in group Cl is to produce a component of ECA.

Keywords

Ulcerative Colitis Ladder Pattern Enterobacterial Common Antigen Clone Restriction Vector EMBL3 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Jann, K., Goldemann, G., Weisgerber, C., Wolf-Ullisch, C., and Kanegasaki, S., 1982. Biosynthesis of the 09 antigen of Escherichia coli: Initial reaction and overall mechanism. Eur. J. Biochem. 127: 157.PubMedCrossRefGoogle Scholar
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    Lew, H. C., Makelä. P. H., Kuhn, H-M., Mayer, H., and Nikaido, H., 1986, Biosynthesis of enterobacterial common antigen requires dTDPglucose pyrophosphorylase determined by a Salmonella montevideo rfe gene. J. Bacteriol. 168: 715.PubMedGoogle Scholar
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • M. Ohta
    • 1
  • N. Kido
    • 1
  • K. Jann
    • 2
  • Y. Arakawa
    • 1
  • T. Komatsu
    • 1
  • H. Ito
    • 1
  • N. Kato
    • 1
  1. 1.Department of BacteriologyNagoya University School of MedicineShowa-ku, Nagoya 466Japan
  2. 2.Max-Planck-Institut für ImmunbiologieFreiburgGermany

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