Abstract
Cyclobutane pyrimidine dimers are preferentially removed from transcriptionally active genes: (i) In Chinese hamster cells which remove only a small fraction of dimers from their total DNA, dimers are efficiently removed from the transcriptionally active dihydrofolate reductase (DHFR) gene and poorly removed from a sequence near the gene (Bohr et al., 1985). (ii) In mouse cells which also remove only a small fraction of dimers from their total DNA, dimers are proficiently removed from the transcriptionally active c-abl gene and poorly removed from the inactive c-mos gene (Madhani et al., 1986). (iii) In repair proficient human cells, dimers are removed more rapidly from the active DHFR gene than from bulk DNA or from the nontranscribed repetitive alpha sequences (Mellon et al., 1986). This work was recently reviewed by Smith (1987).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bohr, V. A., Smith, C. A., Okumoto, D. S., and Hanawalt, P. C., 1985, DNA repair in an active gene: Removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than the genome overall. Cell, 40: 359.
Bohr, V. A., Okumoto, D. S., and Hanawalt, P. C., 1986, Survival of UV-irradiated mammalian cells correlates with efficient DNA repair in an essential gene. Proc. Natl. Acad. Sci. USA, 83:3830.
Crouse, G. F., Leys, E. J., McEwan, R. N., Frayne, E. G. and Kellems, R. E., 1985, Analysis of the mouse DHFR promoter region: existence of a divergently transcribed gene, Mol. Cell. Biol. 5:1847.
Madhani, H. D., Bohr, V. A., and Hanawalt, P. C., 1986, Differential DNA repair in transcriptionally active and inactive proto-oncogenes: cabl and c-mos, Cell, 45: 417.
Mayne, L. V., and Lehmann, A. R., 1982, Failure of RNA synthesis to recover after UV-irradiation: An early defect in cells from individuals with Cockayne’s syndrome and xeroderma pigmentosum, Cancer Research 42: 1473.
Mellon, I., Bohr, V. A., Smith, C. A., and Hanawalt, P. C., 1986, Preferential DNA repair of an active gene in human cells, Proc. Natl. Acad. Sci. USA, 83:8878.
Mellon, I., Spivak, G., and Hanawalt, P. C., 1987, Selective removal of transcription-blocking DNA damage from the transcribed strand of the mammalian DHFR gene, Cell, 51:in press.
Mitchell, P. J., Carothers, A. M., Han, J. H., Harding, J. D., Kas, E., Venolia, L., and Chasin, L. A„ 1986, Multiple transcription start sites, DNase I hypersensitive sites, and an opposite-strand exon in the 5’ region of the CHO DHFR gene, Mol. Cell. Biol. 6:425.
Sauerbier, W. and Hercules, K., 1978, Gene and transcription unit mapping by radiation effects, Ann. Rev. Genet. 12: 329.
Smith, C. A., 1987, DNA repair in specific sequences in mammalian cells, J. Cell Sci. Suppl. 6: 225.
Weintraub, H. and Groudine, M., 1976, Chromosomal subunits in active genes have an altered conformation, Science 193: 848.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Mellon, I., Spivak, G., Hanawalt, P.C. (1989). Selective DNA Repair in the Transcribed Strands of Active Genes in Mammalian Cells. In: Castellani, A. (eds) DNA Damage and Repair. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5016-4_5
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5016-4_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-5018-8
Online ISBN: 978-1-4757-5016-4
eBook Packages: Springer Book Archive