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Visualization and Computer-Assisted Quantification of DNA Modifications in Individual Cells

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DNA Damage and Repair

Abstract

Modification of cellular DNA has been linked with diverging biological effects, such as cell death, cytostatic effects, mutagenesis and carcinogenesis. Important parameters are the pattern and extent of DNA modification, the proliferative activity of the exposed cell, and its capacity to repair relevant DNA damage. The present paper reviews a specific, sensitive, and quantitative immunocytochemical method for the analysis of DNA damage at the single cell level. A number of existing and potential applications in the field of chemical carcinogenesis, chemical mutagenesis, experimental chemotherapy and molecular epidemiology will be discussed.

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Author information

Author notes

  1. C. P. Wild

    Present address: Unit of Mechanisms of Carcinogenesis International Agency for Research on Cancer, Lyon, France

Authors and Affiliations

  1. Division of Chemical Carcinogenesis, The Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis), Amsterdam, The Netherlands

    L. den Engelse, C. P. Wild & E. Scherer

  2. Department of Histochemistry and Cytochemistry, State University of Leiden, Leiden, The Netherlands

    J. S. Ploem

Authors
  1. L. den Engelse
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  2. J. S. Ploem
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  3. C. P. Wild
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  4. E. Scherer
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Editor information

Editors and Affiliations

  1. Department of Environment and Health Protection, ENEA, Rome, Italy

    Amleto Castellani

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© 1989 Springer Science+Business Media New York

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den Engelse, L., Ploem, J.S., Wild, C.P., Scherer, E. (1989). Visualization and Computer-Assisted Quantification of DNA Modifications in Individual Cells. In: Castellani, A. (eds) DNA Damage and Repair. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5016-4_26

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  • DOI: https://doi.org/10.1007/978-1-4757-5016-4_26

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-5018-8

  • Online ISBN: 978-1-4757-5016-4

  • eBook Packages: Springer Book Archive

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