Mammalian DNA Ligase : Studies on the Structure and Function during DNA Replication and Repair

  • M. Mezzina
  • R. H. Elder
  • R. Izzo
  • A. Lisandri
  • J. M. Rossignol
  • U. Bertazzoni
  • A. Sarasin


As part of an enzymological approach to the investigation of proteins involved in the repair of chromosome damage in mammalian cells, we have focused our attention on the enzyme DNA ligase which participates in all the physiological processes involving DNA breakage and rejoining, i.e., DNA repair, recombination and transposition. DNA ligase is also required during DNA replication, since it participates in the replisome complex by catalysing the ligation of Okazaki fragments synthesized in the lagging strand. In all reactions, the enzyme is responsible for covalently joining 3′-hydroxyl to 5′-phosphoryl termini in double-stranded DNA.


Okazaki Fragment Lower Molecular Mass Polypeptide SV40 Infection Lower Molecular Weight Polypeptide Hydroxylapatite Chromatography 
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • M. Mezzina
    • 3
  • R. H. Elder
    • 3
  • R. Izzo
    • 1
  • A. Lisandri
    • 2
  • J. M. Rossignol
    • 3
  • U. Bertazzoni
    • 1
  • A. Sarasin
    • 3
  1. 1.Istituto di Genetica C.N.R.PaviaItaly
  2. 2.Università degli Studi di RomaRomeItaly
  3. 3.Institut de Recherches Scientifiques sur le CancerVillejuifFrance

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