Abstract
As part of an enzymological approach to the investigation of proteins involved in the repair of chromosome damage in mammalian cells, we have focused our attention on the enzyme DNA ligase which participates in all the physiological processes involving DNA breakage and rejoining, i.e., DNA repair, recombination and transposition. DNA ligase is also required during DNA replication, since it participates in the replisome complex by catalysing the ligation of Okazaki fragments synthesized in the lagging strand. In all reactions, the enzyme is responsible for covalently joining 3′-hydroxyl to 5′-phosphoryl termini in double-stranded DNA.
Keywords
- Okazaki Fragment
- Lower Molecular Mass Polypeptide
- SV40 Infection
- Lower Molecular Weight Polypeptide
- Hydroxylapatite Chromatography
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
PAULING, C. and HAMM, L. Proc. Natl. Acad. Sci. USA (1968), 60, 1495–1502.
FAREED, G.C. and RICHARDSON, C.C. Proc. Natl. Acad. Sci. USA (1967) 58, 665–672.
NASMYTH, K.A. Cell (1977) 12, 1109–1120.
FABRE, F. and ROMAN, H. Proc. Natl. Acad. Sci. USA (1979) 76, 4586–4588.
TERAOKA, H. and TSUKADA, K. J. Biol. Chem. (1982) 257 4758–4763.
TERAOKA, H., SUMIKAWA, T., and TSUKADA, K. J. Biol. Chem. (1985) 260 2937–2940.
SODERHALL, S., and LINDAHL, T. Biochem. Biophys. Res. Comm. (1973) 53, 910–916.
TERAOKA, H., SUMIKAWA, T., and TSUKADA, K. J. Biol. Chem. (19861 261 6888–6892.
ARRAND, J.E., WILLIS, A.E., GOLDSMITH, I. and LINDAHL, T. J. Biol. Chem. (1986) 9079–9082.
SODERHALL, S. Nature (1976) 260 640–642.
MEZZINA, M., SUAREZ, H.G., CASSIGNENA, R. and SARASIN, A. Nucleic Acids Res. (1982) 10, 5073–5084.
CREISSEN, D. and SHALL, S. Nature (1982) 296 271–272.
MEZZINA, M., NOCENTINI, S. and SARASIN, A. Biochimie (1982) 64, 743–748.
SIGNORET, M. and DAVID, J.C. Int. Review of Cytology (1986) 103, 249–279.
PEDRALI-NOY, G.C.F., SPADARI, S., CIARROCCHI, G., PEDRINI, A.M. and FALASCHI, A. Eur. J. Biochem. (1973) 39, 343–351.
SPADARI, S. Nucleic Acids Res. (1976) 3, 2155–2166.
TERAOKA, H., TAMURA, S. and TSUKADA, K. Biochim. Biophys. Acta (1979) 563 535–539.
MEZZINA, M., POLITI, N., SARASIN, A. and BERTAZZONI U. Nucleic Acids Res. (1984) 12, 5109–5122
PEDRINI, A.M., NUZZO, F., CIAROCCHI, G., DALPRA, L. and FALASCHI, A. Biochem. Biophys. Res. Comm. (1972) 47 1221–1227
MEZZINA, M. and NOCENTINI, S. Nucleic Acids Res. (1978) 5 4317–4328
MEZZINA, M., FRANCHI, E., IZZO, R., ROSSIGNOL, J.M., BERTAZZONI, U. and SARASIN, A. Biochem. Biophys. Res. Comm. (1985) 132 857–863
PETERSON, T.A., PRAKASH, L., PRAKASH, S., OSLEY, A.M. and REED, S.J. Mol. and Cell. Biol. (1985) 5, 226–235.
MEZZINA, M., ROSSIGNOL, J.M., PHILIPPE, M., IZZO, R., BERTAZZONI, U. and SARASIN, A. Eur. J. Biochem. (1987) 162, 325–332.
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© 1989 Springer Science+Business Media New York
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Mezzina, M. et al. (1989). Mammalian DNA Ligase : Studies on the Structure and Function during DNA Replication and Repair. In: Castellani, A. (eds) DNA Damage and Repair. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5016-4_23
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DOI: https://doi.org/10.1007/978-1-4757-5016-4_23
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