Stable-Flow Free-Boundary (Staflo) Electrophoresis: Three Dimensional Fluid Flow Properties and Applications to Lipoprotein Studies

  • Roger D. Tippetts
  • Howard C. Mel
  • Alexander V. Nichols


Previous papers have described the theory and practice of the stableflow free-boundary (Staflo) method12,13,14 and the application of Staflo electrophoresis or sedimentation to the study of small molecules10,11,14, proteins11, an enzyme substrate reaction15, living cells12,13,14,16, isolated chloroplasts19,21, and lipoproteins27. These studies used analytical and/or separation processes applied to particles dissolved or suspended in a continuously flowing free solution system, in that part of the apparatus called the “Staflo chamber.” The previous analysis was based on the approximation of vertical plug flow within the Staflo chamber, experimentally demonstrated by flow pattern photographs and observations on collection fractions. The possible implications of non-uniform or parabolic flow in the horizontal (thickness) dimension for Staflo migration properties were discussed, but no experimental measurements were available on the true nature of this flow.


Plug Flow Flow Profile Volume Flow Rate Average Residence Time Downstream Position 
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Copyright information

© Springer Science+Business Media New York 1967

Authors and Affiliations

  • Roger D. Tippetts
    • 1
  • Howard C. Mel
    • 1
  • Alexander V. Nichols
    • 1
  1. 1.Division of Medical Physics & Donner LaboratoryUniversity of CaliforniaBerkeleyUSA

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