A Technique for Producing an Artificial Biological Membrane Suitable for Diffusion Studies

  • George B. Delancey
  • Regis R. Stana
  • S. H. Chiang
Conference paper


A technique for investigating the diffusion properties of an artificial biological membrane, protein-lipid-protein, under static and dynamic conditions is discussed. Experimental data on the relative permeability of the membrane to manganous chloride, potassium chloride, sodium chloride, and ammonia in aqueous solutions are presented. The results indicate that manganous chloride and ammonium hydroxide diffuse through the membrane more rapidly than potassium chloride or sodium chloride, while the latter two species exhibit approximately the same overall mass transfer coefficients. It reveals the fact that such an artificial membrane is offering a degree of preferential selectivity which seems to be dependent on the electrical properties rather than on the molecular transfer coefficient of the diffusant. This characteristic is found in conformity with that of natural biological membranes.


Mass Transfer Coefficient Ammonium Hydroxide Droplet Diameter Test Column Lipid Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Chargaff, E., “Lipoproteins”, in “Advances in Protein Chemistry”, Anson, M.L. and Edsall, J.T. (editors), Academic Press, Inc., New York, 1944Google Scholar
  2. 2.
    Danielli, J.F., Journal of Cell. and Comp. Physiology, 7, 393–400, 1935–1936Google Scholar
  3. 3.
    Delancey, G.B., M.S. Thesis, University of Pittsburgh, 1966Google Scholar
  4. 4.
    Engstrom, A., and Finean, J.B., “Biological Ultrastructure”, Academic Press, New York 1958Google Scholar
  5. 5.
    Hawrowitz, F., “The Chemistry and Function of Proteins”, Academic Press, New York, 1963Google Scholar
  6. 6.
    International Critical Tables, Vol. 5Google Scholar
  7. 7.
    Kavanau, J. Lee, “Structure and Function in Biological Membranes”, Vol. I, Holden-Day, Inc., San Francisco, 1965Google Scholar
  8. 8.
    Loewy, A.G., and Siekevitz, P., “Cell Structure and Function”, Hoff, Rinehart, and Winston, Inc., New York, 1963Google Scholar
  9. 9.
    Mercer, E.H., “Cells: Their Structure and Function”, The Natural History Library, Anchor Books, Doubleday and Company, Inc., Garden City, New York, 1962Google Scholar
  10. 10.
    Morrison, R.T., and Boyd, R.N., “Organic Chemistry”, Allyn and Bacon, Boston, 1960Google Scholar
  11. 11.
    Park, C.R., “General Aspects Of Transport Phenomena”, in “Membrane Transport and Metabolism”, Kleinzeller, A., and Kotyk, A. (editors), Academia Press, New York, 1960Google Scholar
  12. 12.
    Paul, J., “Cell Biology: A Currant Summary”, Stanford University Press, Stanford, California, 1964Google Scholar
  13. 13.
    Ponder, E., “Cell Membrane and Its Properties”, in “The Cell”, Brachet, J. and Mivsky, A.E. (editors) Vol. II, Academic Press, New York, 1961Google Scholar
  14. 14.
    Stana, R. R., M. S. Thesis, University of Pittsburgh, 1965.Google Scholar
  15. 15.
    Whittam, R., “The Interdependence of Metabolism and Active Transport”, in “The Cellular Functions of Membrane Transport”, Hoffman, J.F. (editor), Prentice-Hall, Inc., New Jersey, 1964Google Scholar
  16. 16.
    Wittcoff, H., “The Phosphatides”, American Chemical Society Monograph Service, Reinhold Publishing Corp., New York, 1951Google Scholar

Copyright information

© Springer Science+Business Media New York 1967

Authors and Affiliations

  • George B. Delancey
    • 1
  • Regis R. Stana
    • 1
  • S. H. Chiang
    • 1
  1. 1.Department of Chemical EngineeringUniversity of PittsburghPittsburghUSA

Personalised recommendations