Population Balances Based on Cell Number
In Chapter 5, cell population balances are written in terms of a distribution of mass fractions of the total biomass. This allows a direct combination of intracellularly structured models and population models. However, the population balances based on mass fractions do not permit the incorporation into the model of specific events in the cell cycle, and the single-cell models of Section 4.2.4 can therefore not be used in connection with these population balances. Since there are numerous examples that show a direct influence of certain specific events in the cell cycle on the overall culture performance, e.g., the distribution of plasmids to daughter cells on cell division in recombinant cultures, we need to derive a population balance based on cell number to obtain a correct description of these processes.
KeywordsSpecific Growth Rate Dilution Rate Plasmid Copy Number Population Balance Population Balance Equation
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