the ultrastructure of cell membranes can be studied without separating them from other cell constituents. Similarly, their permeability properties and many transport phenomena can be investigated using intact cells or even whole tissue. However, before we can hope to understand the functions of individual membranes in molecular terms, we need to know their precise chemical composition. Procedures must therefore be devised which allow the complete separation of one particular membrane system from the many membranes that constitute the cell. Having achieved this, it is then necessary to show that the isolated membrane is not substantially different from the intact membrane in situ. What we hope to do is to use our knowledge of the isolated membrane in vitro to explain and understand its function and behaviour in vivo.
KeywordsErythrocyte Membrane Membrane Preparation Erythrocyte Ghost Intact Mitochondrion Viral Membrane
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