Enzymatic Catalysis in Bioseparations

  • David G. Rethwisch
  • Jonathan S. Dordick
Chapter
Part of the Topics in Applied Chemistry book series (TAPP)

Abstract

Organic acids and alcohols often constitute the active components of biologically functional molecules including pharmaceuticals, sweeteners, insectides, herbicides, and hormones.1–5 The large-scale production of these molecules generally requires efficient and selective methods of purification.6 Presently, individual peptides are separated from fermentation broths, blood serum, or chemical liquors by reverse phase or ion-exchange high-performance liquid chromatography (HPLC).7 Similarly, racemic mixtures of organic compounds are separated (i.e., optically resolved) into their optically active, biologically functional isomers using chiral-based chromatographic supports,8 or by reaction of the racemate with an optically active co-reactant to form diasteriomers which are more easily separated.9 While these methods have proved to be effective for laboratory-scale separations, they suffer from several limitations for commercial-scale applications. Chromatographic methods are expensive, solvent-intensive, and cannot be run in a continuous fashion. Diasteriomeric separations often require multiple recrystallization steps and consume expensive and often toxic coreactants. Thus, a need has developed for high-volume, low-cost processes for separations of organic acids and alcohols.

Keywords

Liquid Membrane Mandelic Acid Optical Resolution Porcine Pancreatic Lipase Isopropyl Ether 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • David G. Rethwisch
    • 1
  • Jonathan S. Dordick
    • 1
  1. 1.Department of Chemical and Biochemical EngineeringUniversity of IowaIowa CityUSA

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