Hemodynamics, Regional Myocardial Blood Flow, and Sarcoplasmic Reticulum Calcium Uptake in Right Ventricular Hypertrophy and Failure

  • R. K. H. Wyse
  • K. C. Welham
  • M. Jones
  • E. D. Silove
  • M. R. de Leval

Abstract

Either right ventricular hypertrophy (RVH) or failure (RVF) was produced by pulmonary arterial banding in 47 piglets aged 3–6 weeks. When sufficient time was allowed to elapse after banding, RVH was present in 30 and had progressed to RVF in 17. These two groups were compared with 24 control, i.e., normal pigs (C). Animals with RVF differed from RVH and C animals by having reduced cardiac output and clinical signs of failure. Both RVH and RVF had significantly elevated right ventricular peak systolic pressures (RVS), weights (GMRV), and RV/LV systolic pressure ratios (these variables all increased >100% compared with C). The RV (dP/dt)max correlated with RVS in C (r = 0.687, P < 0.001), but this relationship was absent in RVH with higher RVS and GMRV. The RV (dP/dt)max correlated closely with RV blood flow/g per min in C (r = 0.638, P < 0.01) and in RVH (r = 0.462, P < 0.02). Calcium uptake by RV sarcoplasmic reticulum (SR) was diminished in RVH compared to C and further diminished in RVF (38%, P < 0.001). Calcium uptake by LV SR also fell in RVF, suggesting that SR calcium uptake is merely a passive reflection of myocardial function. Our results also suggest that hemodynamic and subcellular changes seen in RVF may be detected during the compensated stage of RVH.

Keywords

Sarcoplasmic Reticulum Calcium Uptake Ventricular Hypertrophy Myocardial Blood Flow Regional Myocardial Blood Flow 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Astrup, P., J0rgensen, K., Andersen, O. S., and Engel, K. 1960. The acid-base metabolism. A new approach. Lancet 1:1035–1039.PubMedCrossRefGoogle Scholar
  2. 2.
    Bray, G. A. 1960. A simple efficient scintillator for counting aqueous solutions in a liquid scintillation counter. Anal Biochem. 1:279–284.CrossRefGoogle Scholar
  3. 3.
    Harigaya, S., and Schwartz, A. 1969. Rate of calcium binding and uptake in normal animal and failing human cardiac muscle. Membrane vesicles (relaxing system) and mitochondria. Circ. Res. 25:781–794.PubMedCrossRefGoogle Scholar
  4. 4.
    Ito, Y., Suko, J., and Chidsey, C. A. 1974. Intracellular calcium and myocardial contractility. V. Calcium uptake of sarcoplasmic reticulum fractions in hypertrophied and failing rabbit hearts. J. Mol Cell Cardiol 6:237–247.PubMedCrossRefGoogle Scholar
  5. 5.
    Katz, A. M., Repke, D. I., Upshaw, J. E., and Polascik, M. A. 1970. Characterisation of dog cardiac microsomes. Use of zonal centrifugation to fractionate fragmented sarcoplasmic reticulum, (Na+ + K+)-activated ATPase and mitochondrial fragments. Biochim. Biophys. Acta 205:473–490.PubMedCrossRefGoogle Scholar
  6. 6.
    Lentz, R. W., Harrison, C. E., Jr., Dewey, J. D., Barnhorst, D. A., Danielson, G. K., and Pluth, J. R. 1978. Functional evaluation of cardiac sarcoplasmic reticulum and mitochondria in human pathologic states. J. Mol Cell Cardiol 10:3–30.PubMedCrossRefGoogle Scholar
  7. 7.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with folin phenol reagent. J. Biol Chem. 193:265–275.PubMedGoogle Scholar
  8. 8.
    Nayler, W. G., Mclnnes, I., Chipperfield, D., Carson, V., and Kurtz, J. B. 1970. Ventricular function and the calcium-accumulating activity of the sarcoplasmic reticulum. J. Mol. Cell Cardiol 1:307–324.PubMedCrossRefGoogle Scholar
  9. 9.
    Penpargkul, S., Repke, D. I., Katz, A. M., and Scheuer, J. 1977. Effect of physical training on calcium transport by rat cardiac sarcoplasmic reticulum. Circ. Res. 40:134–138.PubMedCrossRefGoogle Scholar
  10. 10.
    Sordahl, L. A., McCollum, W. B., Wood, W. G., and Schwartz, A. 1973. Mitochondria and sarcoplasmic reticulum function in cardiac hypertrophy and failure. Am. J. Physiol. 224:497–502.PubMedGoogle Scholar
  11. 11.
    Suko, J., Vogel, J. H. K., and Chidsey, C.A. 1970. Intracellular calcium and myocardial contractility. III. Reduced calcium uptake and ATPase of the sarcoplasmic reticular fraction prepared from chronically failing hearts. Circ. Res. 27:235–247.PubMedCrossRefGoogle Scholar
  12. 12.
    Welham, K. C., Silove, E. D., and Wyse, R. K. H. 1978. Experimental right ventricular hypertrophy and failure in swine. Cardiovasc. Res. 12:61–65.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • R. K. H. Wyse
    • 1
  • K. C. Welham
    • 1
  • M. Jones
    • 1
  • E. D. Silove
    • 1
  • M. R. de Leval
    • 1
  1. 1.Department of Paediatric CardiologyInstitute of Child HealthLondonEngland

Personalised recommendations