Preservation of Oxidative Phosphorylation by Lidocaine in Ischemic and Reperfused Myocardium

  • D. W. Baron
  • M. Sunamori
  • C. E. Harrison


The effect of lidocaine (2 mg/kg bolus, 0.04 mg/kg per min infusion) on ischemic and reperfused myocardial respiration was assessed in 32 dogs, using indices of mitochondrial respiration (ADP:O ratio, state 3 and state 4 respiration, and respiratory control index). Heart rate, left ventricular (LV) pressure, LV dp/dt, cardiac index, epicardial ST segment, and regional myocardial blood flow, using 9 ± 1 μm radiospheres, were measured after 40 min of constriction of the anterior descending coronary artery (N = 16) and after 20 min of reperfusion (N = 16). Results showed that lidocaine increased state 3 respiration and respiratory control in reperfused myocardium (P < 0.05) with both glutamate and succinate-rotenone as substrate. It is concluded that lidocaine improves postischemic mitochondrial oxidative phosphorylation independent of altered hemodynamics or change in myocardial blood flow.


Cardiac Index Myocardial Blood Flow Respiratory Control Reperfused Myocardium Cell Volume Regulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Baron, D. W., Dewey, J. D., and Harrison, C. E. 1980. In vivo NADH fluorescence and its reduction by lidocaine hydrochloride. J. Mol. Cell. Cardiol. 12(Suppl. 1): 12.Google Scholar
  2. 2.
    Bleyaert, A. L., Nemoto, E. M., Safar, P., Stezoski, S. M., Mickell, J. J., Moossy, J., and Rao, G. R. 1978. Thiopental amelioration of brain damage after global ischemia in monkeys. Anesthesiology 49:390–398.PubMedCrossRefGoogle Scholar
  3. 3.
    Boudoulas, H., Karayannacos, P. E., Lewis, R. P., Kakos, G. S., Kilman, J. W., and Vasko, J. S. 1978. Potential effect of lidocaine on ischemic myocardial injury: Experimental and clinical observations. J. Surg. Res. 24:469–476.PubMedCrossRefGoogle Scholar
  4. 4.
    Chance, B., Mela, L., and Harris, E. J. 1968. Interaction of ion movements and local anesthetics in mitochondrial membranes. Fed. Proc. 27:902–906.PubMedGoogle Scholar
  5. 5.
    Cooperstein, S. J., and Lazarow, A. 1951. A microspectrophotometric method for the determination of cytochrome oxidase. J. Biol. Chem. 189:665–670.PubMedGoogle Scholar
  6. 6.
    Harrison, C. E., Jr., Cooper, G. IV, Zujko, K. J., and Coleman, H. N. III. 1972. Myocardial and mitochondrial function in potassium depletion cardiomyopathy. J. Mol. Cell. Cardiol. 4:633–649.PubMedCrossRefGoogle Scholar
  7. 7.
    Heymann, M. A., Payne, B. D., Hoffman, J. I., and Rudolph, A. M. 1977. Blood flow measurements with radionuclide-labeled particles. Prog. Cardiovasc. Dis. 20:55–79.PubMedCrossRefGoogle Scholar
  8. 8.
    Jennings, R. B. 1976. Cell volume regulation in acute myocardial ischemic injury. Acta Med. Scand. [Suppl] 587:83–92.Google Scholar
  9. 9.
    Johnson, C. L., and Schwartz, A. 1969. Some effects of local anesthetics on isolated mitochondria. J. Pharmacol. Exp. Ther. 167:365–373.PubMedGoogle Scholar
  10. 10.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–275.PubMedGoogle Scholar
  11. 11.
    Michenfelder, J. D., Milde, J. H., and Sundt, T. M., Jr. 1976. Cerebral protection by barbiturate anesthesia: Use after middle cerebral artery occlusion in Java monkeys. Arch. Neurol. 33:345–350.PubMedCrossRefGoogle Scholar
  12. 12.
    Mullins, L. J. 1968. From molecules to membranes. Fed. Proc. 27:898–901.PubMedGoogle Scholar
  13. 13.
    Nasser, F. N., Walls, J. T., Edwards, W. D., and Harrison, C. E., Jr. 1980. Lidocaine-induced reduction in size of experimental myocardial infarction. Am. J. Cardiol. 46:967–975.PubMedCrossRefGoogle Scholar
  14. 14.
    Okuda, M., Young, K. R., Jr., and Lefer, A. M. 1976. Localization of glucocorticoid uptake in normal and ischemic myocardial tissue of isolated perfused cat hearts. Circ. Res. 39:640–646.PubMedCrossRefGoogle Scholar
  15. 15.
    Safar, P. N. 1978. Brain resuscitation. In: M. H. Weil and R. J. Henning (eds.), Handbook of Critical Care Medicine, pp. 435–449. Symposia Specialists, Miami.Google Scholar
  16. 16.
    Scarpa, A., and Lindsay, J. G. 1972. Maintenance of energy-linked functions in rat-liver mitochondria aged in the presence of Nupercaine. Eur. J. Biochem. 27:401–407.PubMedCrossRefGoogle Scholar
  17. 17.
    Schaub, R. G., Stewart, G., Strong, M., Ruotolo, R., and Lemole, G. 1977. Reduction of ischemic myocardial damage in the dog by lidocaine infusion. Am. J. Pathol. 87:399–414.PubMedGoogle Scholar
  18. 18.
    Seeman, P. 1972. The membrane actions of anesthetics and tranquilizers. Pharmacol. Rev. 24:583–655.PubMedGoogle Scholar
  19. 19.
    Sinclair, D. M., De Moes, D., Boink, A. B. T. J., and Ruigrok, T. J. C. 1980. A protective effect of thiopentone on hypoxic heart muscle. J. Mol. Cell. Cardiol. 12:225–227.PubMedCrossRefGoogle Scholar
  20. 20.
    Spath, J. A., Jr., Lane, D. L., and Lefer, A. M. 1974. Protective action of methylpred-nisolone on the myocardium during experimental myocardial ischemia in the cat. Circ. Res. 35:44–51.PubMedCrossRefGoogle Scholar
  21. 21.
    Waite, M., and Sisson, P. 1972. Effect of local anesthetics on phospholipases from mitochondria and lysosomes: A probe into the role of the calcium ion in phospholipid hydrolysis. Biochemistry 11:3098–3105.PubMedCrossRefGoogle Scholar
  22. 22.
    Whalen, D. A., Jr., Hamilton, D. G., Ganote, C. E., and Jennings, R. B. 1974. Effect of a transient period of ischemia on myocardial cells. I. Effects on cell volume regulation. Am. J. Pathol. 74:381–397.Google Scholar
  23. 23.
    Yonetani, T. 1967. Cytochrome oxidase: Beef heart. Methods Enzymol. 10:332–335.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • D. W. Baron
    • 1
  • M. Sunamori
    • 1
  • C. E. Harrison
    • 1
  1. 1.Cardiac Muscle Research LaboratoryMayo Clinic and Mayo FoundationRochesterUSA

Personalised recommendations