Effect of Coenzyme Q10 on Hypertrophied Ischemic Myocardium during Aortic Cross Clamping for 2 hr, from the Aspect of Energy Metabolism

  • F. Okamoto
  • K. Karino
  • K. Ohori
  • T. Abe
  • S. Komatsu

Abstract

In order to perform intracardiac repair safely during aortic cross clamping, we designed this study to evaluate the protective effect of coenzyme Q10 (CoQ10) on hypertrophied ischemic myocardium from the aspect of energy metabolism. Six to nine months preceding the study, aortic bandings were carried out on 14 puppies to produce left ventricular hypertrophy (LVH). These dogs with LVH were then subjected to total cardiopulmonary bypass and were evenly divided into control and CoQ10-treated groups (10 mg/kg of intravenous administration plus 1 mg/kg per hr of intracoronary injection). Myocardial ischemia was induced by aortic cross clamping for 2 hr under moderate systemic hypothermia. The results indicated that the administration of CoQio had a protective effect on hypertrophied ischemic myocardium, since depletion of high-energy phosphate (HEP) was uniformly prevented, and accumulation of lactate was simultaneously decreased during the 2 hr of aortic cross clamping. On the other hand, there were marked exhaustion of HEP and rapid increase in lactate following the 2 hr of ischemia in the control group, these being much more predominant in the subendocardial layer.

Keywords

Left Ventricular Hypertrophy Creatine Phosphate Aortic Banding Intracardiac Repair Subendocardial Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Buckberg, G. D. 1979. A proposed “solution” to the cardioplegic controversy, J. Thorac. Cardiovasc. Surg. 77:803–815.PubMedGoogle Scholar
  2. 2.
    Cooley, D. A., Reul, G. J., and Wukash, D. C. 1972. Ischemic contracture of the heart: “Stone heart.” Am. J. Cardiol. 29:575–577.PubMedCrossRefGoogle Scholar
  3. 3.
    Crane, F. L., Hatefi, Y., Lester, R. L., and Widmer, C. 1957. Isolation of a quinone from beef heart mitochondria. Biochim. Biophys. Acta 25:220–221.PubMedCrossRefGoogle Scholar
  4. 4.
    Engelman, R. M., Rousou, J. H., O’Donoghue, M. J., Longo, F., and Dobbs, W. A. 1980. A comparison of intermittent and continuous arrest for prolonged hypothermic cardioplegia. Ann. Thorac. Surg. 29:217–223.PubMedCrossRefGoogle Scholar
  5. 5.
    Ernster, L. 1977. Facts and ideas about the functions of coenzyme Q10 in mitochondria. In: K. Folkers and Y. Yamamura (eds.), Biomedical and Clinical Aspects of Coenzyme Q, pp. 15–21. Elsevier, Amsterdam.Google Scholar
  6. 6.
    Folkers, K., Watanabe, T., and Kaji, M. 1977. Critique of coenzyme Q in biochemical and biomedical research and in ten years of clinical research on cardiovascular disease. J. Mol. Med. 2:431–460.Google Scholar
  7. 7.
    Guy, C., and Eliot, R. S. 1970. The subendocardium of the left ventricle, a physiologic enigma. Chest 58:555–556.PubMedCrossRefGoogle Scholar
  8. 8.
    Hearse, D. J., Stewart, D. A., and Chain, E.B. 1974. Recovery from cardiac bypass and elective cardiac arrest. The metabolic consequences of various cardioplegic procedures in the isolated rat heart. Circ. Res. 35:448–457.PubMedCrossRefGoogle Scholar
  9. 9.
    Kirk, E. S., and Honig, C. R. 1964. An experimental and theoretical analysis of myocardial tissue pressure. Am. J. Physiol. 207:361–367.PubMedGoogle Scholar
  10. 10.
    Kuby, S. A., and Naltman, E. A. 1962. In: P. D. Boyer, H. A. Lardy, and K. Myrbäcck (eds.), The Enzymes, Vol. VI, pp. 515–603. Academic Press, New York.Google Scholar
  11. 11.
    Roe, B. B., Hutchinson, J. C., Fishman, N. H., Ullyot, D. J., and Smith, D. L. 1977. Myocardial protection with cold, ischemic, potassium-induced cardioplegia. J. Thorac. Cardiovasc. Surg. 73:366–370.PubMedGoogle Scholar
  12. 12.
    Samokhvalov, G. I., Obolnikova, F. A., and Naifakh, S. A. 1977. Biochemical aspects of administration of hexahydroubequinone-4 and derivative of reduced form thereof. In: K. Folkers and Y. Yamamura (eds.), Biomedical and Clinical Aspects of Coenzyme Q, pp. 201–217. Elsevier, Amsterdam.Google Scholar
  13. 13.
    Tatsukawa, Y., Dohi, Y., Yamada, K., and Kawasaki, T. 1979. The role of coenzyme Q10 for preservation of the rat kidney: An model experiment for kidney transplantation. Life Sci. 24:1309–1314.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • F. Okamoto
    • 1
  • K. Karino
    • 1
  • K. Ohori
    • 1
  • T. Abe
    • 1
  • S. Komatsu
    • 1
  1. 1.Department of Thoracic and Cardiovascular SurgerySapporo Medical CollegeSapporo 060Japan

Personalised recommendations