Reflections on What Makes the Heart Grow

  • F. Kölbel
  • V. Schreiber
  • J. Štěpán
  • T. Přibyl
  • I. Gregorová


Under conditions of experimental cardiac overload and hypertrophy in rats, a di-goxinlike immunoreactivity appears in their serum which is correlated with cardiac growth. It is hypothesized that this is caused by the presence of an endogenous cardiotropic factor displaying cross immunoreactivity with digoxin. Additional evidence of the existence of the putative cardiotropic factor is provided by the finding that the sera of rats with cardiac overload displaying digoxinlike immunoreactivity stimulate the multiplication of rat cardiac myocytes in the tissue culture. This factor may be an adrenal steroid different from corticosterone and aldosterone. The name endocardin or endocardiotonin for this substance is suggested.


Aortic Stenosis Abdominal Aorta Heart Weight Adrenal Weight Digitalis Glycoside 
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  1. 1.
    Beznak, M. 1964. Hormonal influences in regulation of cardiac performance. Cire. Res. 14–15(Suppl. 2): 141–152.Google Scholar
  2. 2.
    Caldarera, C. M., Guarnieri, C., Clo, C., Ferrari, R., and Casti, A. 1979. Early biochemical events of myocardial hypertrophy In: P. Padieu and J.-P. Didier (eds.), 3eme Reunion de la Section Europeenne De l’ISHR, pp. 125–138. Faculté de Medecine de Dijon, Dijon.Google Scholar
  3. 3.
    Drost, R. H., Plomp, T. A., Teunissen, A. J., Maas, A. H. J., and Maes, R. A. A. 1977. A comparative study of the homogenous enzyme immunoassay (EMIT) and two radioimmunoassays (RIA’s) for digoxin. Clin. Chim. Acta 79:557–567.PubMedCrossRefGoogle Scholar
  4. 4.
    Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics 11:1–42.CrossRefGoogle Scholar
  5. 5.
    Fízel, A., and Fízelová, A. 1972. Participation of catecholamines and cortical hormones in the myocardial metabolic adaptation to mechanical overload of the heart. In: E. Bajusz, and G. Rona (eds.) Myocardiology Vol. 1, pp. 814–827. Urban & Schwarzenberg, Munich, Berlin, Vienna.Google Scholar
  6. 6.
    Kölbel, F. 1978. Biochemické mechanismy vzniku hypertrofie levé srdeční komory [Biochemical mechanisms of the induction of left ventricular hypertrophy.] Cesk. Fysiol. 27:523–530.Google Scholar
  7. 7.
    Kölbel, F., Kapitola, J., Schreiberová, O., and Kölblová, V. 1970. Influence of adrenalectomy on the effects of isoproterenol in the rat. Physiol. Bohemoslov. 19:281–289.PubMedGoogle Scholar
  8. 8.
    Kölbel, F., Kapitola, J., Šonka, J., and Schreiberová, O. 1972. Hormonal and humoral modulation of the effects of isoproterenol. In: P.-Y. Hatt (ed.), Les Surcharges Cardiaques, pp. 39–47. INSERM, Paris.Google Scholar
  9. 9.
    Kölbel, F., Mommaerts, W. F. H. M., Kölblová, V., and Vančura, P. 1970. Cardiac muscle and liver ribosomes of the rat: The influence of laparotomy and laparotomy combined with adrenalectomy. Experientia 26:361–362.PubMedCrossRefGoogle Scholar
  10. 10.
    Limas, C. J., and Chan-Stier, C. 1978. Myocardial chromatin activation in experimental hyperthyroidism in rats. Circ. Shock 42:311–322.Google Scholar
  11. 11.
    Mattingly, D. 1962. A simple fluorometric method for the estimation of free 11-hydroxy-corticoids in human plasma. J. Clin. Pathol. 15:374–385.PubMedCrossRefGoogle Scholar
  12. 12.
    Morris, B. J., Davis, J. O., Zatzman, M. L., and Williams, G. M. 1977. The renin angiotensin aldosterone system in rabbits with congestive heart failure produced by aortic constriction. Circ. Res. 40:275–286.PubMedCrossRefGoogle Scholar
  13. 13.
    Rubenstein, K. E., Schneider, R. S., and Ullman, E. F. 1972. Biochem. Biophys. Res. Commun. 47:846–851.PubMedCrossRefGoogle Scholar
  14. 14.
    Schreiber, V., Kölbel, F., Horký, K., and Štěpán, J. 1980. Hormonální faktory v růstových reakcích srdečního svalu [Hormonal factors in growth reactions of heart muscle] Cesk. Fysiol. 29:489–500.PubMedGoogle Scholar
  15. 15.
    Schreiber, V., Kölbel, F., and Štěpán, J. 1980. Zdánlivá (?) imunoreaktivita digoxinu v séru krys se srde&čním přetížením. K otázce endogenního kardiotonika (endokardinu) [Apparent (?) immunoreactivity of digoxin in the sera of rats with cardiac overload—on the problem of endogenous cardiotonic (endocardin)]. Cas. Lek. Cesk. 119:768–770.PubMedGoogle Scholar
  16. 16.
    Schreiber, V., Kölbel, F., Štěpán, J., Gregorová, I., and Přibyl, T. 1980. Digoxin-like immunoreactivity in the serum of rats with cardiac overload. J. Mol. Cell. Cardiol. 75:107–110.Google Scholar
  17. 17.
    Schreiber, V., Štěpán, J., Gregorová, I., Kölbel, F., Přibyl, T., Jahodová, J., and Janovská, J. 1980. Digoxinu podobná imunoreaktivita v seru hyperthyreoidních krys se srdeční hy-pertrofií není způsobena aldosteronem [Digoxinlike immunoreactivity in the serum of hy-perthyroid rats with cardiac hypertrophy is not caused by aldosterone]. Sb. Lek. 82:305–308.PubMedGoogle Scholar
  18. 18.
    Schreiber, V., Štěpán, J., Kölbel, F., Přibyl, T., Jahodová, J., and Kubová, V. 1980. Failure of the aldosterone antagonist spironolactone to inhibit myocardial hypertrophy produced by experimental hyperthyroidism and accompanied by “apparent” digoxin immunoreactivity in the blood. Physiol. Bohemoslov. 29:577–579.PubMedGoogle Scholar
  19. 19.
    Sharma, V. K., and Banerjee, S. P. 1978. Specific 3H-ouabain binding to rat heart and skeletal muscle: Effects of thyroidectomy. Mol. Pharmacol. 14:122–129.PubMedGoogle Scholar
  20. 20.
    Turto, H., and Lindy, S. 1973. Digitoxin treatment and experimental cardiac hypertrophy in the rat. Cardiovasc. Res. 7:482–487.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • F. Kölbel
    • 1
  • V. Schreiber
    • 1
  • J. Štěpán
    • 1
  • T. Přibyl
    • 1
  • I. Gregorová
    • 1
  1. 1.Third Medical Department and Laboratory for Endocrinology and Metabolism, Faculty of MedicineCharles UniversityPragueCzechoslovakia

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