Ultrastructural, Functional, and Metabolic Correlates in the Ischemic Rat Heart

Effects of Free Fatty Acid
  • D. Feuvray


A study correlating functional, metabolic, and ultrastructural changes in the ischemic myocardium was conducted on isolated working rat hearts, both in the presence and absence of fatty acid. Glucose alone (11 mM) or glucose plus palmitic acid (1.5 mM) were used as metabolic substrates. A 60-min period of whole-heart ischemia resulted in a more dramatic morphological alteration in those hearts receiving palmitate than in those receiving no palmitate. In ischemic hearts receiving palmitate, intramitochondrial amorphous densities of both rounded and elongated types were observed. These densities did not develop in hearts receiving glucose alone over the same period of ischemia. Such morphological alterations were associated with a more severe deterioration of mechanical function in the presence of palmitate. Biochemical determinations of fatty acid derivatives showed increased tissue levels of acyl esters of CoA and carnitine in ischemic hearts, but levels of long-chain acyl carnitine were much higher in those ischemic hearts receiving palmitate. Furthermore, from the data obtained on isolated mitochondria, it appeared that the mitochondrial level of long-chain acyl carnitine was approximately four times higher in the ischemic hearts receiving palmitate than in those receiving no palmitate. This great rise in mitochondrial levels of long-chain acyl carnitine correlated with modifications of the mitochondrial structure and with the appearance of amorphous densities.


Ischemic Heart Coronary Artery Ligation Mitochondrial Level Acyl Carnitine Fine Structural Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Feuvray, D. 1981. Structural, functional and metabolic correlates in ischemic hearts: Effects of substrates. Am. J. Physiol. 240:H391–398.PubMedGoogle Scholar
  2. 2.
    Feuvray, D., and Plouet, J. 1981. Relationship between structure and fatty acid metabolism in mitochondria isolated from ischemic rat hearts. Circ. Res. 48:740–747.PubMedCrossRefGoogle Scholar
  3. 3.
    Garland, P. B., Shepherd, D., and Yates, D. W. 1965. Steady state concentrations of coenzyme A, acetyl coenzyme A and long-chain fatty acyl coenzyme A in rat liver mitochondria oxidizing palmitate. Biochem. J. 97:587–594.PubMedGoogle Scholar
  4. 4.
    Idell-Wenger, J. A., Grotyohann, L. W., and Neely, J. R. 1978. Coenzyme A and carnitine distribution in normal and ischemic hearts. J. Biol. Chem. 253:4310–4318.PubMedGoogle Scholar
  5. 5.
    Jennings, R. B., and Ganote, C.E. 1972. Ultrastructural changes in acute myocardial ischemia. In: M. F. Oliver, D. G. Julian, and K. W. Donald (eds.), Effects of Acute Ischaemia on Myocardial Function, pp. 50–74. Churchill Livingstone, Edinburgh.Google Scholar
  6. 6.
    Jennings, R. B., and Ganote, C. E. 1976. Mitochondrial structure and function in acute myocardial ischemic injury. Circ. Res. 38:80–91.Google Scholar
  7. 7.
    Jennings, R. B., and Herdson, P. B. 1965. Fine structural changes in myocardial ischemic injury. Arch. Pathol. 79:135–143.PubMedGoogle Scholar
  8. 8.
    Leiris, J. de, and Feuvray, D. 1977. Ischaemia-induced damaged in the working rat heart preparation: The effect of perfusate substrate composition upon subendocardial ultrastructure of the ischaemic left ventricular wall. J. Mol. Cell. Cardiol. 9:365–373.PubMedCrossRefGoogle Scholar
  9. 9.
    Leiris, J. de, and Opie, L. H. 1978. Effect of substrates and of coronary artery ligation on mechanical performance and on release of lactate dehydrogenase and creatine Phosphokinase in isolated working rat hearts. Cardiovasc. Res. 12:585–596.PubMedCrossRefGoogle Scholar
  10. 10.
    Leiris, J. de, Opie, L. H., and Feuvray, D. 1975. Effect of substrate on enzyme release and electron microscopic appearances after coronary artery ligation in isolated rat heart. Acta Med. Scand. 587:137–139.Google Scholar
  11. 11.
    Liedtke, A. J., Nellis, S., and Neely, J. R. 1978. Effects of excess free fatty acids on mechanical and metabolic function in normal and ischemic myocardium in swine. Cire. Res. 43:652–661.CrossRefGoogle Scholar
  12. 12.
    McGarry, J. D., and Foster, D. W. 1976. An improved and simplified radioisotope assay for the determination of free and esterified carnitine. J. Lipid Res. 17:277–281.PubMedGoogle Scholar
  13. 13.
    Neely, J. R., Liebermeister, H., Battersby, E. J., and Morgan, H. E. 1967. Effect of pressure development on oxygen consumption by isolated rat heart. Am. J. Physiol. 212:804–814.PubMedGoogle Scholar
  14. 14.
    Neely, J. R., Rovetto, M. J., Whitmer, J. T., and Morgan, H. E. 1973. Effects of ischemia on ventricular function and metabolism in the isolated working rat heart. Am. J. Physiol. 225:651–658.PubMedGoogle Scholar
  15. 15.
    Opie, L. H. 1972. Metabolic response during impending myocardial infarction. I. Relevance of studies of glucose and fatty acid metabolism in animals. Circulation 45:483–489.PubMedCrossRefGoogle Scholar
  16. 16.
    Pitts, B. J. R., Tate, C. A., Van Winkle, W. B., Wood, J. M., and Entman, M. L. 1978. Palmityl carnitine inhibition of the calcium pump in cardiac sarcoplasmic reticulum. A possible role in myocardial ischemia. Life Sci. 23:391–402.PubMedCrossRefGoogle Scholar
  17. 17.
    Schaper, J., Mulch, J., Winkler, B., and Schaper, W. 1979. Ultrastructural, functional and biochemical criteria for estimation of reversibility of ischemic injury: A study on the effects of global ischemia on the isolated dog heart. J. Mol. Cell. Cardiol. 11:521–541.PubMedCrossRefGoogle Scholar
  18. 18.
    Shug, A. L., Shrago, E., Bittar, N., Folts, J. D., and Kokes, J. R. 1975. Acyl CoA inhibition of adenine nucleotide translocation in ischemic myocardium. Am. J. Physiol. 228:689–692.PubMedGoogle Scholar
  19. 19.
    Sordahl, L. A., Johnson, C, Blailock, Z. R., and Schwartz, A. 1971. In: A. Schwartz (ed.), Methods in Pharmacology Vol. 1, pp. 247–286. Appleton Century Crofts, New York.CrossRefGoogle Scholar
  20. 20.
    Whitmer, J. T., Idell-Wenger, J. A., Rovetto, M. J., and Neely, J. R. 1978. Control of fatty acid metabolism in ischemic and hypoxic hearts, J. Biol. Chem. 253:4305–4309.PubMedGoogle Scholar
  21. 21.
    Wood, J. M., Busch, B., Pitts, B. J. R., and Schwartz, A. 1977. Inhibition of bovine heart Na+, K+-ATPase by palmityl carnitine and palmityl CoA. Biochem. Biophys. Res. Commun. 74:677–684.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • D. Feuvray
    • 1
  1. 1.Laboratory of Comparative PhysiologyUniversity of Paris-SudOrsayFrance

Personalised recommendations