Release of Purine Nucleosides and Oxypurines from the Isolated Perfused Rat Heart

  • J. W. de Jong
  • E. Harmsen
  • P. P. de Tombe
  • E. Keijzer


In the ischemic heart, high-energy phosphates are rapidly broken down. We studied the release of AMP catabolites from the isolated perfused rat heart which was temporarily made ischemic or anoxic. We measured the concentration of purine nucleosides and oxypurines with a novel high-pressure liquid chromatographic technique. The postischemic working heart released adenosine, inosine, hypoxanthine, and also substantial amounts of xanthine. The latter could indicate that xanthine oxidase is present in rat heart. Further evidence for the myocardial occurrence of this enzyme was obtained from experiments with hearts perfused retrogradely with allopurinol, an inhibitor of xanthine oxidase. This drug greatly enhanced the release of hypoxanthine, both during normoxic and anoxic perfusions. We conclude that xanthine oxidase could play an essential role in the myocardial breakdown of AMP catabolites.


Xanthine Oxidase Coronary Flow Purine Nucleoside Modify Tyrode Solution Aortic Outflow Tract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Amory, N., Delbarre, F., and Auscher, C. 1978. Localization of xanthine oxidase in human and rat tissues. A histochemical study. C. R. Acad. Sci. [D] (Paris) 287:1007–1009.Google Scholar
  2. 2.
    De Jong, J. W. 1972. Phosphorylation and deamination of adenosine by the isolated perfused rat heart. Biochim. Biophys. Acta286:252–259.PubMedCrossRefGoogle Scholar
  3. 3.
    De Jong, J. W. 1979. Biochemistry of acutely ischemic myocardium. In: W. Schaper (ed.), The Pathophysiology of Myocardial Perfusion, pp. 719–750. Elsevier/North-Holland, Amsterdam.Google Scholar
  4. 4.
    De Jong, J. W., Verdouw, P. D., and Remme, W. J. 1977. Myocardial nucleoside and carbohydrate metabolism and hemodynamics during partial occlusion and reperfusion of pig coronary artery. J. Mol. Cell. Cardiol. 9:297–312.PubMedCrossRefGoogle Scholar
  5. 5.
    Gandhi, M. P. S., and Ahuja, S. P. 1979. Absorption of xanthine oxidase from the intestines of rats and rabbits and its role in initiation of atherosclerosis. Zentralbl. Veterinaermed. 26:635–642.CrossRefGoogle Scholar
  6. 6.
    Harmsen, E., De Jong, J. W., Keijzer, E., and Uitendaal, M. P. 1980. Assay of whole blood (hypo)xanthine and its precursors. J. Mol. Cell. Cardiol. 12(Suppl. 1):54.Google Scholar
  7. 7.
    Maguire, M. H., Lukas, M. C, and Rettie, J. F. 1972. Adenine nucleotide salvage synthesis in the rat heart; pathways of adenosine salvage. Biochim. Biophys. Acta262:108–115.PubMedCrossRefGoogle Scholar
  8. 8.
    Neely, J. R., and Rovetto, M. J. 1975. Techniques for perfusing isolated rat hearts. Methods Enzymol. 39:43–60.PubMedCrossRefGoogle Scholar
  9. 9.
    Olsson, R. A. 1970. Changes in content of purine nucleoside in canine myocardium during coronary occlusion. Circ. Res. 26:301–306.PubMedCrossRefGoogle Scholar
  10. 10.
    Ramboer, C. R. H. 1969. A sensitive and nonradioactive assay for serum and tissue xanthine oxidase. J. Lab. Clin. Med. 74:828–835.PubMedGoogle Scholar
  11. 11.
    Remme, W. J., De Jong, J. W., and Verdouw, P. D. 1977. Effects of pacing-induced myocardial ischemia on hypoxanthine efflux from the human heart. Am. J. Cardiol. 40:55–62.PubMedCrossRefGoogle Scholar
  12. 12.
    Stam, H., and De Jong, J. W. 1977. Sephadex-induced reduction of coronary flow in the isolated rat heart: A model for ischemic heart disease. J. Mol. Cell. Cardiol. 9:633–650.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • J. W. de Jong
    • 1
  • E. Harmsen
    • 1
  • P. P. de Tombe
    • 1
  • E. Keijzer
    • 1
  1. 1.Cardiochemical Laboratory, ThoraxcenterErasmus University RotterdamRotterdamThe Netherlands

Personalised recommendations